Type 3 Secretion Translocators Spontaneously Assemble a Hexadecameric Transmembrane Complex.

The Journal of Biological Chemistry
Fabian B RomanoAlejandro P Heuck

Abstract

A type 3 secretion system is used by many bacterial pathogens to inject proteins into eukaryotic cells. Pathogens insert a translocon complex into the target eukaryotic membrane by secreting two proteins known as translocators. How these translocators form a translocon in the lipid bilayer and why both proteins are required remains elusive. Pseudomonas aeruginosa translocators PopB and PopD insert pores into membranes forming homo- or hetero-complexes of undetermined stoichiometry. Single-molecule fluorescence photobleaching experiments revealed that PopD formed mostly hexameric structures in membranes, whereas PopB displayed a bi-modal distribution with 6 and 12 subunits peaks. However, individually the proteins are not functional for effector translocation. We have found that when added together, the translocators formed distinct hetero-complexes containing 8 PopB and 8 PopD molecules. Thus, the interaction between PopB and PopD guide the assembly of a unique hetero-oligomer in membranes.

References

Jan 1, 1985·Biophysical Journal·L K Tamm, H M McConnell
Oct 1, 1995·Biophysical Journal·L W Runnels, S F Scarlata
Mar 20, 1999·Infection and Immunity·A J VallisD W Frank
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·X ZhuangS Chu
Nov 1, 2001·Annual Review of Cell and Developmental Biology·J E Galán
Jan 10, 2002·Journal of the American Chemical Society·Fredrik BergströmLennart B A Johansson
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Guy R Cornelis
May 24, 2003·Annals of the New York Academy of Sciences·Shunji KayaYutaro Hayashi
Nov 6, 2004·Science·Thomas C MarlovitsVinzenz M Unger
Jun 18, 2005·The Journal of Infectious Diseases·Julien GoureIna Attree
Oct 17, 2006·Nature Reviews. Microbiology·Guy R Cornelis
Nov 30, 2006·Biochemistry·Guruvasuthevan R ThuduppathyR Blake Hill
Dec 1, 2006·Nature·Jorge E Galán, Hans Wolf-Watz
Mar 21, 2007·Molecular Microbiology·Andreas K J VeenendaalAriel J Blocker
May 16, 2007·Chembiochem : a European Journal of Chemical Biology·Somes K DasHagan Bayley
Feb 9, 2008·Current Opinion in Structural Biology·Trevor F MoraesNatalie Cj Strynadka
Apr 24, 2008·Molecular Microbiology·C A MuellerG R Cornelis
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
May 15, 2010·Methods in Cell Biology·Jennifer L Ross, Ram Dixit
Dec 25, 2010·The FEBS Journal·Pierre-Jean MatteïAndréa Dessen
Mar 10, 2011·Science·Oliver Schraidt, Thomas C Marlovits
May 18, 2011·Biophysical Journal·Juan Daniel Díaz-ValenciaJennifer L Ross
Oct 19, 2011·The Journal of Biological Chemistry·Nicolas GroulxRikard Blunck
May 16, 2012·The Protein Journal·Supratim DeySaumen Datta
Oct 19, 2013·Biochimica Et Biophysica Acta·Joel SelkrigTrevor Lithgow

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Citations

Apr 11, 2017·Nature Reviews. Microbiology·Wanyin DengB Brett Finlay
Jul 14, 2019·Protein Science : a Publication of the Protein Society·Supratim DeyRoberto N De Guzman
Sep 25, 2016·Biological Chemistry·Susann ZilkenatSamuel Wagner
Sep 10, 2021·PLoS Pathogens·Brian C RussoMarcia B Goldberg

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