Type and capacity of glucose transport influences succinate yield in two-stage cultivations

Microbial Cell Factories
L KyselovaK Bettenbrock

Abstract

Glucose is the main carbon source of E. coli and a typical substrate in production processes. The main glucose uptake system is the glucose specific phosphotransferase system (Glc-PTS). The PTS couples glucose uptake with its phosphorylation. This is achieved by the concomitant conversion of phosphoenolpyruvate (PEP) to pyruvate. The Glc-PTS is hence unfavorable for the production of succinate as this product is derived from PEP. We studied, in a systematic manner, the effect of knocking out the Glc-PTS and of replacing it with the glucose facilitator (Glf) of Zymomonas mobilis on succinate yield and productivity. For this study a set of strains derived from MG1655, carrying deletions of ackA-pta, adhE and ldhA that prevent the synthesis of competing fermentation products, were constructed and tested in two-stage cultivations. The data show that inactivation of the Glc-PTS achieved a considerable increase in succinate yield and productivity. On the other hand, aerobic growth of this strain on glucose was strongly decreased. Expression of the alternative glucose transporter, Glf, in this strain enhanced aerobic growth but productivity and yield under anaerobic conditions were slightly decreased. This decrease in succinate yield ...Continue Reading

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Citations

Sep 29, 2020·Frontiers in Bioengineering and Biotechnology·Ying LiQian Wang
Dec 21, 2021·Molecular Systems Biology·Simon BoeckerSteffen Klamt

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Methods Mentioned

BETA
PCR
gene knock-outs

Software Mentioned

Gurobi
CobraToolbox
Cobra Toolbox
MATLAB

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