PMID: 9525633Apr 3, 1998Paper

Type C retrovirus released from porcine primary peripheral blood mononuclear cells infects human cells

Journal of Virology
C A WilsonP Burd

Abstract

As part of the evaluation of porcine cells, tissues, and organs intended for transplantation into humans, we investigated the conditions required to induce expression and release of porcine endogenous retrovirus (PoEV) from primary cells. Pigs contain endogenous retroviral sequences encoding infectious retrovirus, yet little is known about the conditions required to activate the expression and release of PoEV from primary cells. We show here that mitogenic activation of peripheral blood mononuclear cells (PBMC) isolated from the National Institutes of Health (NIH) miniature pig and the Yucatan pig resulted in the activation and release of an infectious type C retrovirus. Coculture of activated porcine PBMC with pig or human cell lines resulted in the transfer and expression of PoEV-specific sequences and the establishment of a productive infection. Sequence comparison of portions of the PoEV pol gene expressed in pig cell lines productively infected with virus derived from NIH miniature pig and Yucatan pig PBMC revealed marked similarity, suggesting that one or a few loci may be capable of being activated to yield an infectious virus. These findings demonstrate that the presence of endogenous viruses in source animals needs to ...Continue Reading

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Citations

Jan 29, 2000·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·S L NybergR H Wiesner
Nov 18, 2000·Antimicrobial Agents and Chemotherapy·S K PowellE Otto
Nov 17, 2001·Journal of Virology·C HerringL Scobie
May 7, 2002·Journal of Virology·Jun-Heon LeeChris Moran
Jun 11, 2003·Xenotransplantation·Maritza C McIntyreMatiza C McIntyre
Oct 30, 2004·Annals of Medicine·Roumiana S Boneva, Thomas M Folks
Nov 27, 2004·Journal of Virology·Ian HarrisonJonathan P Stoye
Nov 19, 2005·Expert Review of Medical Devices·Thorsten Walles
Feb 14, 2007·Reviews in Medical Virology·Eric L Delwart
Nov 8, 2007·Reviews in Medical Virology·Derrick LouzRob C Hoeben
Feb 14, 2009·Molecules and Cells·Jae-Won HuhHeui-Soo Kim
Jan 29, 2010·In Vitro Cellular & Developmental Biology. Animal·Peng ZhangHong Bu
Jul 14, 2011·Journal of Cardiovascular Translational Research·M K Sewell-LoftinW David Merryman
Nov 12, 2016·World Journal for Pediatric & Congenital Heart Surgery·Toshiharu Shinoka, Hideki Miyachi
Dec 12, 2019·Journal of Tissue Engineering and Regenerative Medicine·Claudia BilodeauMartin Post
Feb 3, 1999·Annals of the New York Academy of Sciences·J P StoyeR A Weiss
Mar 12, 2002·Journal of Cardiac Surgery·J A Fishman
Dec 10, 1999·Journal of Virology·C A WilsonM J Federspiel
Nov 11, 2003·Genome Génome / Conseil National De Recherches Canada·Ruhul H KuddusJohn J Fung
Mar 12, 2002·Journal of Cardiac Surgery·J Z AppelD K Cooper

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