Type I collagen-induced YAP nuclear expression promotes primary cilia growth and contributes to cell migration in confluent mouse embryo fibroblast 3T3-L1 cells

Molecular and Cellular Biochemistry
Qian XuTakashi Ikejima

Abstract

The extracellular matrix (ECM) is a major biomechanical environment for all cells in vivo, and tightly controls wound healing and cancer progression. Type I collagen (Col I) is the most abundant component in ECM and plays an essential role for cell motility control and migration beyond structural support. Our previous results showed that Col I increased the length of primary cilia and the expression of primary cilia-associated proteins in 3T3-L1 cells. The Hippo/YAP pathway serves as a major integrator of cell surface-mediated signals and regulates key processes for the development and maintenance of tissue functions. In this study, we investigated the role of Hippo/YAP signaling in primary cilia growth of cells cultured on Col I-coated plate, as well as the potential link between primary cilia and migration. At 2-day post-confluence, YAP localization in the nucleus was dramatically increased when the cells were cultured on Col I-coated plate, accompanied by cilia growth. YAP inhibitor verteporfin repressed the growth of primary cilia as well as the expressions of ciliogenesis-associated proteins in confluent 3T3-L1 cells cultured on Col I-coated plate. Moreover, knockdown of either YAP or IFT88, one of the ciliogenesis-associa...Continue Reading

References

May 22, 2003·Annals of Biomedical Engineering·Charles W Patrick, Xuemei Wu
Aug 16, 2006·American Journal of Human Genetics·Anneke I den HollanderFrans P M Cremers
Aug 25, 2006·Journal of the American Society of Nephrology : JASN·Laurie A SmithOxana Ibraghimov-Beskrovnaya
Jan 24, 2007·Traffic·Søren T ChristensenPeter Satir
Jan 30, 2010·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Linda SchneiderSøren T Christensen
May 5, 2010·Genes & Development·Bin ZhaoKun-Liang Guan
Jun 17, 2010·Advances in Experimental Medicine and Biology·Andrea Becchetti, Annarosa Arcangeli
Jun 10, 2011·Nature·Sirio DupontStefano Piccolo
Aug 13, 2011·Development·Ken-Ichi WadaHiroshi Sasaki
Dec 20, 2011·Differentiation; Research in Biological Diversity·Prachee Avasthi, Wallace F Marshall
Feb 22, 2012·The Journal of Cell Biology·Pengfei LuZena Werb
Mar 26, 2013·Methods in Enzymology·Søren T ChristensenPeter Satir
Nov 20, 2013·International Journal of Oncology·Shin-Hyung ParkYung Hyun Choi
Dec 18, 2013·Nature Reviews. Drug Discovery·Randy Johnson, Georg Halder
Oct 8, 2014·Physiological Reviews·Stefano PiccoloMichelangelo Cordenonsi
Mar 23, 2015·Biochemical and Biophysical Research Communications·Ji Hyun ShinDong-Hyung Cho
Jun 6, 2015·Trends in Cell Biology·Carsten Gram HansenKun-Liang Guan

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Methods Mentioned

BETA
protein assay
electrophoresis
PCR
fluorescence microscopy
confocal microscopy

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Image Pro Plus
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