Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family.

The Journal of Biological Chemistry
H IshiharaY Oka

Abstract

Type I phosphatidylinositol 4-phosphate (PtdIns(4)P) 5-kinases (PIP5K) catalyze the synthesis of phosphatidylinositol 4, 5-bisphosphate, an essential lipid molecule in various cellular processes. Here, we report the cloning of the third member (PIP5Kgamma) and the characterization of members of the type I PIP5K family. Type I PIP5Kgamma has two alternative splicing forms, migrating at 87 and 90 kDa on SDS-polyacrylamide gel electrophoresis. The amino acid sequence of the central portion of this isoform shows approximately 80% identity with those of the alpha and beta isoforms. Northern blot analysis revealed that the gamma isoform is highly expressed in the brain, lung, and kidneys. Among three isoforms, the beta isoform has the greatest Vmax value for the PtdIns(4)P kinase activity and the gamma isoform is most markedly stimulated by phosphatidic acid. By analyzing deletion mutants of the three isoforms, the minimal kinase core sequence of these isoforms were determined as an approximately 380-amino acid region. In addition, carboxyl-terminal regions of the beta and gamma isoforms were found to confer the greatest Vmax value and the highest phosphatidic acid sensitivity, respectively. It was also discovered that lysine 138 in ...Continue Reading

References

Nov 1, 1990·Trends in Biochemical Sciences·M SarasteA Wittinghofer
Sep 21, 1989·Nature·M J Berridge, R F Irvine
Jul 28, 1995·The Journal of Biological Chemistry·K F ToliasC L Carpenter
Aug 1, 1995·The Biochemical Journal·N DivechaR F Irvine
Feb 17, 1995·The Journal of Biological Chemistry·I V Boronenkov, R A Anderson
Feb 1, 1994·Protein Science : a Publication of the Protein Society· MadhusudanJ M Sowadski
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·S MiyakeI Saito
Apr 26, 1996·The Journal of Biological Chemistry·M BuntingS M Prescott
May 17, 1996·The Journal of Biological Chemistry·T NakagawaH Kondo
Sep 27, 1996·The Journal of Biological Chemistry·H IshiharaY Oka
Dec 20, 1996·The Journal of Biological Chemistry·J C Loijens, R A Anderson
Mar 21, 1997·The Journal of Biological Chemistry·Y ShibasakiY Yazaki
Jul 1, 1997·Molecular and Cellular Biology·T SakisakaT Takenawa
Aug 29, 1997·The Journal of Biological Chemistry·L E RamehL C Cantley

❮ Previous
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Citations

Jan 25, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Paschal A Oude WeerninkMartina Schmidt
May 24, 2007·Pflügers Archiv : European journal of physiology·Yuntao S Mao, Helen L Yin
Jun 24, 2004·Cellular Signalling·Bai LuoMatthew K Topham
Jun 8, 1999·Chemistry and Physics of Lipids·K F Tolias, L C Cantley
Sep 2, 1999·Advances in Enzyme Regulation·C L CarpenterJ Hartwig
Mar 17, 2004·Brain Research. Gene Expression Patterns·Yosuke AkibaHisatake Kondo
Oct 20, 2007·Nature Protocols·Zachary A KnightKevan M Shokat
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·W A Snedden, E Blumwald
Sep 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yanfeng WangCharles S Abrams
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yasuko NodaNobutaka Hirokawa
Jul 14, 2010·The Journal of Biological Chemistry·Laura A Volpicelli-DaleyPietro De Camilli
Jan 11, 2011·The Journal of Biological Chemistry·Yung-Luen YuMien-Chie Hung
Jan 10, 2013·The Journal of Biological Chemistry·Tingting ZhuWei Zou
Mar 17, 2009·The Journal of Biological Chemistry·James R ThiemanLinton M Traub
Aug 27, 2005·Molecular Biology of the Cell·P D AroraC A McCulloch
Jul 12, 2007·Genome Research·Daria V BabushokHaig H Kazazian
Sep 23, 2006·Science·Byung-Chang SuhBertil Hille
Jun 8, 2001·Annual Review of Biochemistry·B VanhaesebroeckM D Waterfield
Sep 20, 2012·Journal of Lipid Research·Akua N RoachGuangwei Du
Oct 16, 2009·Journal of Lipid Research·Norma M GiustoMónica G Ilincheta de Boschero
Sep 11, 2007·Biological & Pharmaceutical Bulletin·Yasunori KanahoTakeaki Yokozeki
Feb 24, 2012·PloS One·Małgorzata DudkiewiczKrzysztof Pawłowski

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