Type II pneumocytes release chemoattractant activity for monocytes constitutively

The American Journal of Physiology
S KoyamaT Izumi

Abstract

In the present investigation, we determined whether A549 cells, a type II pneumocyte cell line, might release mediators that are responsible for monocyte chemoattractant activity (MCA) constitutively. To test this hypothesis, A549 cell supernatant fluids were harvested and evaluated for monocyte chemotaxis. A549 cell supernatant fluids showed MCA in a time-dependent manner (P < 0.001). Checkerboard analysis of 24- and 72-h supernatant fluids showed that the activity was chemokinetic rather than chemotactic. Partial characterization of 24- and 72-h supernatant fluids revealed that the mediator was composed of lipid-soluble activity that was blocked by lipoxygenase inhibitors and trypsin-sensitive activity blocked by cycloheximide. Molecular sieve column chromatography identified four molecular weight peaks. Two of four peaks were blocked by anti-monocyte chemoattractant protein-1 (MCP-1) and anti-transforming growth factor-beta (TGF-beta) polyclonal antibodies. MCP-1 and TGF-beta were detected by enzyme-linked immunosorbent assay. Leukotriene B4 (LTB4) receptor antagonist attenuated the lowest-molecular-weight peak chemotactic response, and the concentration of LTB4 was high enough for chemotactic activity. These findings sugges...Continue Reading

Citations

Apr 6, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·S KoyamaT Izumi
Mar 11, 2003·American Journal of Respiratory Cell and Molecular Biology·Hiroki NumanamiRichard A Robbins
Jun 11, 2005·American Journal of Respiratory Cell and Molecular Biology·Stefan HammerschmidtHubert Wirtz
Mar 15, 2013·American Journal of Physiology. Cell Physiology·Jacob Bak HolmIan Henry Lambert
May 10, 2005·American Journal of Respiratory and Critical Care Medicine·Karim Vermaelen, Romain Pauwels
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·S RosseauJ Lohmeyer

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