Type II protein kinase A regulates CFTR in airway, pancreatic, and intestinal cells

The American Journal of Physiology
W K SteagallM L Drumm

Abstract

The type of protein kinase A (PKA) responsible for cystic fibrosis transmembrane conductance regulator (CFTR) activation was determined with adenosine 3', 5'-cyclic monophosphate analogs capable of selectively activating type I or type II PKA. The type II-selective pair stimulated chloride efflux in airway, pancreatic, and colonic epithelial cells; the type I-selective pair only stimulated a calcium-dependent efflux in airway cells. The type II-selective analogs activated larger increases in CFTR-mediated current than did the type I-selective analogs. Measurement of soluble PKA activity demonstrated similar levels stimulated by type I- and type II-selective analogs, creating an apparent paradox regarding PKA activity and current generated. Also, addition of forskolin after the type I-selective analogs resulted in an increase in current; little increase was seen after the type II-selective analogs. Measurement of insoluble PKA activity stimulated by the analogs resolved this paradox. Type II-selective analogs stimulated three times as much insoluble PKA activity as the type I-selective pair, indicating that differential activation of PKA in cellular compartments is important in CFTR regulation.

References

Jan 1, 1992·Pflügers Archiv : European journal of physiology·F BecqE Hollande
Aug 1, 1990·The American Journal of Physiology·C J VenglarikR A Frizzell
Jan 1, 1990·Annual Review of Biochemistry·S S TaylorW Yonemoto
Jan 1, 1988·Methods in Enzymology·S J BeebeJ D Corbin
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·T C HwangD C Gadsby
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·F BecqJ W Hanrahan
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D O QuissellL M Deisher
Jan 1, 1994·Annual Review of Physiology·S H Francis, J D Corbin
Apr 1, 1994·Journal of Molecular Endocrinology·B J Whitehouse, D R Abayasekara
Oct 1, 1993·The American Journal of Physiology·K E Barrett
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·T J KelleyM L Drumm

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Citations

Dec 30, 1999·American Journal of Respiratory Cell and Molecular Biology·W K SteagallT J Kelley
Dec 2, 2004·Experimental Physiology·John Adam Best, P M Quinton
Jul 16, 2008·Current Gastroenterology Reports·John D Long, Roy C Orlando
Aug 10, 2011·Nature Reviews. Gastroenterology & Hepatology·José Altamirano, Ramón Bataller
Feb 9, 2000·American Journal of Physiology. Cell Physiology·P HuangM J Stutts
Mar 2, 2006·Digestive Diseases·Soren V SiegmundManfred V Singer
May 26, 2009·Current Gastroenterology Reports·Lori A Orlando, Roy C Orlando
Dec 15, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Akos SzucsGábor Varga
Jan 31, 2004·Scandinavian Journal of Gastroenterology·M Bortolotti
May 16, 2007·Pharmacogenetics and Genomics·Wendy K SteagallJoel Moss
Nov 14, 2019·International Journal of Molecular Sciences·Mikio Hayashi
May 16, 2002·American Journal of Veterinary Research·Tamara Vetro WidenhouseAlfred M Merritt
Mar 12, 2016·Pflügers Archiv : European journal of physiology·M HayashiH Matsuda
Sep 29, 2000·Pharmacology & Therapeutics·F SchwedeB Jastorff
Nov 8, 2002·Biochimica Et Biophysica Acta·W S Marshall, T D Singer

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