Type II secretion system of Pseudomonas aeruginosa: in vivo evidence of a significant role in death due to lung infection

The Journal of Infectious Diseases
Jeevan JyotReuben Ramphal

Abstract

The role of toxins secreted by the type II secretion system (T2SS) of Pseudomonas aeruginosa during lung infection has been uncertain despite decades of research. Using a model of pneumonia in Toll-like receptor (TLR) 2,4(-/-) mice, we reexamined the role of the T2SS system. Flagellin-deficient mutants of P. aeruginosa, with mutations in the T2SS and/or T3SS, were used to infect mice. Mice were followed up for survival, with some killed at different intervals to study bacterial clearance, inflammatory responses, and lung pathology. Strains carrying either secretion system were lethal for mice. Double mutants were avirulent. The T3SS(+) strains killed mice within a day, and the T2SS(+) strains killed them later. Mice infected with a strain that had only the T2SS were unable to eradicate the organism from the lungs, whereas those infected with a T2SS-T3SS double deletion were able to clear this mutant. Death caused by the T2SS(+) strain was accompanied by a >50-fold increase in bacterial counts and higher numbers of viable intracellular bacteria. The T2SS of P. aeruginosa may play a role in death from pneumonia, but its action is delayed. These data suggest that antitoxin strategies against this organism will require measures aga...Continue Reading

References

Apr 3, 2012·Nature Reviews. Microbiology·Konstantin V KorotkovWim G J Hol
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Delphyne DescampsJean-Michel Sallenave
May 3, 2012·MBio·Amy T Y YeungRobert E W Hancock
Jan 28, 2014·PloS One·Mercedes Ortega-GonzálezDaddaoua Abdelali
Dec 22, 2015·International Journal of Medical Microbiology : IJMM·Maitrayee ChatterjeeRaja Biswas
Aug 16, 2011·Microbes and Infection·Elise G LavoieBarbara I Kazmierczak
Mar 8, 2017·Infection and Immunity·Nicholas P Cianciotto, Richard C White
Nov 10, 2018·Environmental Microbiology·Anne LorenzSusanne Häussler
Aug 8, 2015·The European Respiratory Journal·Antoine GuillonMustapha Si-Tahar
Nov 8, 2019·British Journal of Haematology·A Valance WashingtonAngelia Gibson
Mar 5, 2017·Postȩpy higieny i medycyny doświadczalnej·Fairoz Al-WrafyAndrzej Gamian

Citations

Sep 1, 1992·FEMS Microbiology Reviews·J TommassenAndrée Lazdunski
Oct 1, 1981·Infection and Immunity·P A SokolW J Iglewski
Jun 1, 1982·Canadian Journal of Microbiology·B WeberC B Saelinger
Apr 1, 1996·Chest·S Crouch BrewerK V Leeper
Oct 1, 1996·FEMS Immunology and Medical Microbiology·L L LaichalkT J Standiford
Sep 8, 1998·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·L RavozzoniC Buono
May 24, 2001·The Journal of Infectious Diseases·A Roy-BurmanJ P Wiener-Kronish
Sep 11, 2001·Journal of Medical Microbiology·M J SchultzT van der Poll
May 3, 2002·Molecular Microbiology·Geneviève BallAlain Filloux
Mar 20, 2003·Journal of Bacteriology·Victoria E WagnerBarbara H Iglewski
Mar 29, 2003·Archives of Microbiology·Wilbert Bitter
Nov 24, 2004·Infection and Immunity·Ciara M Shaver, Alan R Hauser
Aug 24, 2005·The Journal of Biological Chemistry·Manuelle QuinaudIna Attrée
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Reuben RamphalMichel Chignard
Oct 12, 2005·Trends in Microbiology·Nicholas P Cianciotto
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin N CouperEleanor M Riley
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Reuben RamphalMichel Chignard
Jan 27, 2009·Current Opinion in Microbiology·Joanne Engel, Priya Balachandran
Jun 19, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Grasiella M de C VenturaMustapha Si-Tahar
Jan 27, 2010·Infection and Immunity·Jared T MuenzerRichard S Hotchkiss

Related Concepts

Pseudomonas Infections
Gene Expression Regulation, Bacterial
Pseudomonas aeruginosa (antigen)
Systemic Inflammatory Response Syndrome
Lung
Toll-like receptor 4
Tlr4 protein, mouse
Flagellin
Infectious Disease of Lung
Toxin

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.