Type III intermediate filament peripherin inhibits neuritogenesis in type II spiral ganglion neurons in vitro.

Neuroscience Letters
Meagan BarclayGary D Housley

Abstract

Peripherin, a type III intermediate filament protein, forms part of the cytoskeleton in a subset of neurons, most of which have peripheral fibre projections. Studies suggest a role for peripherin in axon outgrowth and regeneration, but evidence for this in sensory and brain tissues is limited. The exclusive expression of peripherin in a sub-population of primary auditory neurons, the type II spiral ganglion neurons (SGN) prompted our investigation of the effect of peripherin gene deletion (pphKO) on these neurons. We used confocal immunofluorescence to examine the establishment of the innervation of the cochlear outer hair cells by the type II SGN neurites in vivo and in vitro, in wildtype (WT) and pphKO mice, in the first postnatal week. The distribution of the type II SGN nerve fibres was normal in pphKO cochleae. However, using P1 spiral ganglion explants under culture conditions where the majority of neurites were derived from type II SGN, pphKO resulted in increased numbers of neurites/explant compared to WT controls. Type II SGN neurites from pphKO explants extended approximately double the distance of WT neurites, and had reduced complexity based on greater distance between turning points. Addition of brain-derived neuro...Continue Reading

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Sep 28, 2007·Histochemistry and Cell Biology·Meagan BarclayGary D Housley
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Citations

Nov 20, 2010·Cell and Tissue Research·Wei LiuHelge Rask-Andersen
Mar 1, 2012·PloS One·Marisa ZallocchiDominic Cosgrove
Oct 23, 2013·Neurología : publicación oficial de la Sociedad Española de Neurología·U Gomez-PinedoA G García
Aug 23, 2016·Cells·Flora Guerra, Cecilia Bucci
Apr 20, 2011·Journal of Cell Science·Claire L HyderJohn E Eriksson
Aug 6, 2021·Laryngo- rhino- otologie·Athanasia Warnecke, Anja Giesemann

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