Type III Secretion Effector VopQ of Vibrio parahaemolyticus Modulates Central Carbon Metabolism in Epithelial Cells.

MSphere
Anh Quoc NguyenAkira Takahashi

Abstract

Vibrio parahaemolyticus is a Gram-negative halophilic pathogen that frequently causes acute gastroenteritis and occasional wound infection. V. parahaemolyticus contains several virulence factors, including type III secretion systems (T3SSs) and thermostable direct hemolysin (TDH). In particular, T3SS1 is a potent cytotoxic inducer, and T3SS2 is essential for causing acute gastroenteritis. Although much is known about manipulation of host signaling transductions by the V. parahaemolyticus effector, little is known about the host metabolomic changes modulated by V. parahaemolyticus To address this knowledge gap, we performed a metabolomic analysis of the epithelial cells during V. parahaemolyticus infection using capillary electrophoresis-time of flight mass spectrometry (CE-TOF/MS). Our results revealed significant metabolomic perturbations upon V. parahaemolyticus infection. Moreover, we identified that T3SS1's VopQ effector was responsible for inducing the significant metabolic changes in the infected cells. The VopQ effector dramatically altered the host cell's glycolytic, tricarboxylic acid cycle (TCA), and amino acid metabolisms. VopQ effector disrupted host cell redox homeostasis by depleting cellular glutathione and subse...Continue Reading

References

Jan 1, 1992·Journal of Nutritional Science and Vitaminology·A Meister
Jan 7, 2000·Microbial Pathogenesis·T NomuraK Okamoto
May 24, 2000·The Journal of Infectious Diseases·N A DanielsL Slutsker
Oct 27, 2004·Infection and Immunity·Kwon-Sam ParkTakeshi Honda
Jan 24, 2006·Infection and Immunity·Takahiro OnoTakeshi Honda
May 5, 2006·BMC Genomics·Catherine C HurleyE Fidelma Boyd
Mar 30, 2007·Journal of Internal Medicine·M P Fink
Jul 2, 2008·BMC Microbiology·E Fidelma BoydMichelle A Parent
Sep 18, 2008·Molecular Aspects of Medicine·Henry Jay FormanAlessandra Rinna
Jul 25, 2009·Molecular Microbiology·Dara L BurdetteKim Orth
Dec 24, 2010·The Journal of Infectious Diseases·Takaaki ShimohataAkira Takahashi
Nov 19, 2011·Cold Spring Harbor Symposia on Quantitative Biology·I MorK H Vousden
Apr 27, 2012·Bioanalysis·Tiziana PacchiarottaOleg A Mayboroda
Apr 27, 2012·Journal of Clinical Microbiology·Jessica L JonesAngelo DePaola
Sep 22, 2012·Biochimica Et Biophysica Acta·Shelly C Lu
Apr 2, 2013·Metabolomics : Official Journal of the Metabolomic Society·Kenjiro KamiMasaru Tomita
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anju SreelathaVincent J Starai
Jan 24, 2014·Case Reports in Infectious Diseases·Asim AhmadMichael Maniaci
Mar 19, 2014·The Journal of Experimental Medicine·Meghan A Wynosky-DolfiIgor E Brodsky
Mar 25, 2014·Annual Review of Immunology·Kirthana Ganeshan, Ajay Chawla
Jun 30, 2014·Advances in Cancer Research·Yilin LiuJoseph J Barycki
Dec 3, 2014·Proceedings of the National Academy of Sciences of the United States of America·Anju SreelathaVincent J Starai
Jun 30, 2015·Cell Reports·Jong-Seok MoonAugustine M K Choi
Oct 7, 2015·Infection and Immunity·Chinh T Q NguyenAnthony W Maresso
Nov 8, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Johji NomuraNathalie Busso
Aug 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Luigi VezzulliCarla Pruzzo
Oct 26, 2016·Nature Communications·Min XieDaolin Tang
Oct 28, 2017·Frontiers in Physiology·Lonneke M JanssenDuijghuijsenJaap Keijer
Mar 15, 2018·Frontiers in Immunology·Wenkai RenYulong Yin
May 16, 2018·Nucleic Acids Research·Jasmine ChongJianguo Xia
Mar 10, 2019·Immunology·Qiuli YangGuangwei Liu

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Citations

Sep 10, 2020·Electrophoresis·Wei Zhang, Rawi Ramautar
Mar 26, 2022·Microbiology·Fatima R Ulhuq, Giuseppina Mariano

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Methods Mentioned

BETA
PCR
electrophoresis
PCA

Software Mentioned

Metabolomics
GraphPad
VopQ
Mass Hunter
MSEA
MetaboAnalyst Suite
MetaboAnalyst
GraphPad Prism

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