Type III Transforming Growth Factor-β Receptor RNA Interference Enhances Transforming Growth Factor β 3-Induced Chondrogenesis Signaling in Human Mesenchymal Stem Cells

Stem Cells International
Shuhui ZhengCaixia Xu

Abstract

The type III transforming growth factor-β (TGF-β) receptor (TβRIII), a coreceptor of the TGF-β superfamily, is known to bind TGF-βs and regulate TGF-β signaling. However, the regulatory roles of TβRIII in TGF-β-induced mesenchymal stem cell (MSC) chondrogenesis have not been explored. The present study examined the effect of TβRIII RNA interference (RNAi) on TGF-β3-induced human MSC (hMSC) chondrogenesis and possible signal mechanisms. A lentiviral expression vector containing TβRIII small interfering RNA (siRNA) (SiTβRIII) or a control siRNA (SiNC) gene was constructed and infected into hMSCs. The cells were cultured in chondrogenic medium containing TGF-β3 or control medium. TβRIII RNAi significantly enhanced TGF-β3-induced chondrogenic differentiation of hMSCs, the ratio of type II (TβRII) to type I (TβRI) TGF-β receptors, and phosphorylation levels of Smad2/3 as compared with cells infected with SiNC. An inhibitor of the TGF-β signal, SB431542, not only inhibited TβRIII RNAi-stimulated TGF-β3-mediated Smad2/3 phosphorylation but also inhibited the effects of TβRIII RNAi on TGF-β3-induced chondrogenic differentiation. These results demonstrate that TβRIII RNAi enhances TGF-β3-induced chondrogenic differentiation in hMSCs by ...Continue Reading

References

Feb 27, 2008·Carcinogenesis·Nadine HempelGerard C Blobe
May 12, 2009·Expert Opinion on Biological Therapy·Quen Oak TangEleftherios Tsiridis
May 10, 2011·Molecular and Cellular Endocrinology·Maree Bilandzic, Kaye L Stenvers
Jul 6, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Caixia XuJiaan Zhou
Jan 27, 2015·Molecular Biotechnology·Christian AlbrechtSylvia Nürnberger
Aug 14, 2015·Molecular Biology of the Cell·Keren TazatYoav I Henis
Sep 17, 2015·Current Reviews in Musculoskeletal Medicine·Shinichi Yoshiya, Aman Dhawan
Aug 5, 2016·Journal of Clinical Orthopaedics and Trauma·Sung Woo HuhSeok Jung Kim
Jan 3, 2018·Acta Biochimica Et Biophysica Sinica·Joachim NickelThomas D Mueller

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Citations

Aug 10, 2019·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Caixia XuPeiqiang Su
Jul 18, 2020·Frontiers in Bioengineering and Biotechnology·René RothweilerMartin James Stoddart
Oct 30, 2020·Cancers·Vivian Weiwen XuePatrick Ming-Kuen Tang

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Methods Mentioned

BETA
PCR
transfection
Protein Assay
fluorescence microscopy

Software Mentioned

SPSS

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