Type V collagen regulates the expression of apoptotic and stress response genes by breast cancer cells

Journal of Cellular Physiology
Claudio Luparello, Rosalia Sirchia

Abstract

Type V collagen is a "minor" component of normal human breast stroma, which is subjected to over-deposition in cases of ductal infiltrating carcinoma (DIC). We reported that, if used as a culture substrate for the DIC cell line 8701-BC, it exhibited poorly-adhesive properties and restrained the proliferative and motile behavior of the cell subpopulation able to attach onto it. Moreover, this collagen species was able to trigger DNA fragmentation and impair survival of 8701-BC cells. In this study, we have extended our investigation with the aim to obtain further evidence that the death induced by type V collagen was of the apoptotic type by (i) microscopic detection and quantitation of Apoptag-labeled cells, (ii) analysis of the expression levels of selected genes coding for apoptosis-linked factors, caspases, and stress-response proteins by conventional and semi-quantitative multiplex PCR, and (iii) evaluation of the extent of caspase activation by chromogenic assay. We report here that type V collagen is able to determine an increase in the percentage of Apoptag-positive cells, to up-regulate Bcl-xS, Bad, Dap kinase, hsf-1, mthsp75, caspase-1, -5, -8, -9, and -14, whilst down-regulating Bcl-2, Bcl-xbeta, and hsp60. Treatment ...Continue Reading

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Citations

Jun 26, 2012·Analytical and Bioanalytical Chemistry·Alessandra LongoClaudio Luparello
Nov 22, 2007·Breast Cancer Research and Treatment·Claudio LuparelloBruna Lo Sasso
Oct 11, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Tong Liu, Jian Zhang
Feb 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·David J SugarbakerRaphael Bueno
May 24, 2008·Carcinogenesis·Hector Guillen-AhlersVictoria A Ploplis
Sep 17, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Caterina CasanoClaudio Luparello
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Mar 2, 2021·Pathology, Research and Practice·Roberta Goncalves MarangoniNatalino H Yoshinari
Aug 31, 2021·Frontiers in Oncology·Tabatha Gutierrez PrietoVera Luiza Capelozzi

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