PMID: 3771648Oct 1, 1986Paper

Type VII collagen is a major structural component of anchoring fibrils

The Journal of Cell Biology
L Y SakaiR E Burgeson

Abstract

Anchoring fibrils are specialized fibrous structures found in the subbasal lamina underlying epithelia of several external tissues. Based upon their sensitivity to collagenase and the similarity in banding pattern to artificially created segment-long spacing crystallites (SLS) of collagens, several authors have suggested that anchoring fibrils are lateral aggregates of collagenous macromolecules. We recently reported the similarity in length and banding pattern of anchoring fibrils to type VII collagen SLS crystallites. We now report the construction and characterization of a murine monoclonal antibody specific for type VII collagen. The epitope identified by this antibody has been mapped to the carboxyl terminus of the major helical domain of this molecule. The presence of type VII collagen as detected by indirect immunofluorescence in a variety of tissues corresponds exactly with ultrastructural observations of anchoring fibrils. Ultrastructural immunolocalization of type VII collagen using a 5-nm colloidal gold-conjugated second antibody demonstrates metal deposition upon anchoring fibrils at both ends of these structures, as predicted by the location of the epitope on type VII collagen. Type VII collagen is synthesized by p...Continue Reading

References

Apr 1, 1976·European Journal of Immunology·G KöhlerC Milstein
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R E BurgesonD W Hollister
Oct 14, 1967·Nature·M StefaniniL Zamboni
Aug 1, 1969·The Journal of Cell Biology·R R Bruns
Jan 1, 1982·Collagen and Related Research·D W HollisterR E Burgeson
Jan 1, 1984·Differentiation; Research in Biological Diversity·H HessleE Engvall
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H BentzR E Burgeson
Nov 1, 1982·Collagen and Related Research·K von Der Mark, M Ocalan
Jul 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·H EAGLE
Mar 1, 1957·Proceedings of the Society for Experimental Biology and Medicine·J FOGH, R O LUND

❮ Previous
Next ❯

Citations

Jun 1, 1997·The Journal of Pathology·I HopkinsonK G Harding
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Colognato, P D Yurchenco
Jul 9, 1999·Journal of Biomedical Materials Research·M D EvansJ G Steele
Oct 31, 2000·Microscopy Research and Technique·K A McGowan, M P Marinkovich
Aug 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M P MarinkovichR E Burgeson
Jun 1, 1994·Microscopy Research and Technique·H J Merker
Feb 1, 1990·Cell and Tissue Research·M RittigJ Mollenhauer
Jun 1, 1993·Cell and Tissue Research·P Köpf-Maier, C Schröter-Kermani
Jan 1, 1994·Archives of Dermatological Research·R A Eady, M G Dunnill
Jan 1, 1989·Archives of Dermatological Research·N OkadaM L Steinberg
Jan 1, 1989·Archives of Dermatological Research·Y HoriguchiJ R Couchman
Nov 1, 1989·The Histochemical Journal·F T BosmanM Havenith
Dec 1, 1990·The Histochemical Journal·J P CleutjensF T Bosman
Sep 29, 1995·Experientia·P Simon-AssmannP Simo
Sep 1, 1996·The Histochemical Journal·I VirtanenV P Lehto
May 1, 1996·The Histochemical Journal·G MagroM Lopes
Jun 1, 1996·The Histochemical Journal·J LohiI Virtanen
Feb 1, 1996·Archives of Dermatological Research·J R McMillan, R A Eady
Oct 1, 1996·In Vitro Cellular & Developmental Biology. Animal·K JanssonA Haegerstrand
Jun 20, 2007·Archives of Dermatological Research·Yannis DaleziosGeorges Chalepakis
Dec 17, 2002·Atherosclerosis·Gabriele A M PlenzWolfgang Völker
Jul 12, 2001·Otolaryngologic Clinics of North America·H P Gaboriau, C S Murakami
Oct 18, 2001·Clinics in Dermatology·R F GhohestaniJ Uitto
Nov 14, 2001·Clinics in Dermatology·D Hallel-HalevyD T Woodley
Mar 1, 1994·Journal of Voice : Official Journal of the Voice Foundation·S D GrayP Harding
Jan 4, 2001·Journal of Dermatological Science·T NishiyamaR E Burgeson
May 3, 2003·Journal of Dermatological Science·James R McMillanHiroshi Shimizu
Jun 24, 1999·Journal of Dermatological Science·D Zillikens
Jan 29, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M TsunenagaT Nishiyama
Aug 3, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·S GrässelP Bruckner
Sep 28, 2000·Micron : the International Research and Review Journal for Microscopy·B Eyden, M Tzaphlidou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Related Papers

Annals of the New York Academy of Sciences
R E BurgesonD R Keene
Proceedings of the National Academy of Sciences of the United States of America
M G ParenteJ Uitto
Proceedings of the National Academy of Sciences of the United States of America
H BentzR E Burgeson
© 2021 Meta ULC. All rights reserved