PMID: 11331207May 2, 2001Paper

Type VIII collagen: heterotrimeric chain association

The International Journal of Biochemistry & Cell Biology
C IllidgeA Shuttleworth

Abstract

Two chains, alpha1(VIII) and alpha2(VIII), have been described for type VIII collagen. Early work suggested that these chains were present in a 2:1 ratio, although recent work has shown that homotrimers can form and predominate in some tissues. In order to address the question of whether the alpha1(VIII) and alpha2(VIII) chains could co-polymerise we made a shortened alpha1(VIII) chain and expressed this with full length alpha2(VIII) chain in an in vitro translation system supplemented with semi-permeabilised cells. Heterotrimers containing either two or one alpha2(VIII) were evident. Interestingly, a point mutation in the NC1 domain of the alpha1(VIII) chain abrogated trimer formation. In addition we were able to demonstrate chain association of the alpha1(X) chain of type X collagen with the shortened alpha1(VIII) chain. Variations in chain association were seen when altered ratios of message were used. These results demonstrate the importance of the NC1 domain in chain association and suggest that gene expression regulates the composition and function of type VIII collagen by varying chain composition.

References

Nov 1, 1996·The Biochemical Journal·J R MacBeathC A Shuttleworth
Aug 15, 1998·The Journal of Biological Chemistry·C IllidgeA Shuttleworth
Feb 25, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·N S GreenhillP F Davis

❮ Previous
Next ❯

Citations

Jul 25, 2015·Cell and Tissue Research·Dawiyat MassoudiStephane D Galiacy
Aug 12, 2009·Biochemical and Biophysical Research Communications·Jonathan M PetersonG William Wong
Nov 28, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·John M Gansner, Jonathan D Gitlin
Oct 25, 2011·Experimental Eye Research·Clare KelliherAlbert S Jun
Apr 30, 2009·Diabetes·Ulrike HopferGunter Wolf
Apr 20, 2005·Advances in Protein Chemistry·Carlo Knupp, John M Squire
Jan 27, 2018·Cardiovascular Research·Kerry WadeySarah George
Aug 16, 2003·The Journal of Biological Chemistry·Audrey McAlindenDavid J S Hulmes
Mar 3, 2004·The Journal of Biological Chemistry·Simon StephanCay M Kielty
Feb 18, 2021·Experimental Eye Research·Lauren J JeangEdgar M Espana
Jul 15, 2020·Experimental Eye Research·Edgar M Espana, David E Birk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Matrix Biology : Journal of the International Society for Matrix Biology
Nicholas S GreenhillPaul F Davis
Biochemical and Biophysical Research Communications
N D Rosenblum
The International Journal of Biochemistry & Cell Biology
C A Shuttleworth
© 2021 Meta ULC. All rights reserved