Type X collagen gene expression is transiently up-regulated by retinoic acid treatment in chick chondrocyte cultures

Experimental Cell Research
H F Oettinger, M Pacifici

Abstract

During endochondral ossification, resting and proliferating chondrocytes mature into hypertrophic chondrocytes that initiate synthesis of type X collagen. The mechanisms regulating the differential expression of type X collagen gene were examined in confluent Day 12 secondary cultures of chick vertebral chondrocytes in monolayer treated with the vitamin A analog retinoic acid (RA). Preliminary results showed that major effects of RA on chondrocyte gene expression occurred between 24 and 48 h of treatment. Thus in subsequent experiments cultures were treated for 24, 30, 36, 42, 48, 72, 96, and 120 h. Total RNAs were isolated and analyzed by hybridization with 32P-labeled plasmid probes coding for five matrix macromolecules including type X collagen. We found that the steady-state levels of mRNAs for the large keratan sulfate/chondroitin sulfate proteoglycan (KS:CS-PG) core protein and type II collagen decreased several fold between 24 and 48 h of treatment compared to untreated cells, and remained low with further treatment. In sharp contrast, the level of type X collagen mRNA increased threefold by 42 h of treatment; thereafter it began to decrease and reached minimal levels by 72-120 h of treatment. The changes in steady-state...Continue Reading

References

Sep 8, 1975·Biochimica Et Biophysica Acta·S Lohmander, A Hjerpe
Jan 1, 1989·Connective Tissue Research·S L AdamsM Pacifici
Dec 1, 1987·Molecular and Cellular Biology·P A Norton, R O Hynes
Nov 1, 1988·Trends in Genetics : TIG·S Green, P Chambon
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S SaiM L Tanzer
May 25, 1984·Nucleic Acids Research·M F YoungM E Sobel
Oct 1, 1980·Experimental Cell Research·M PacificiF Tato

❮ Previous
Next ❯

Citations

Oct 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M PacificiC M Chuong
Jan 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S M SmithJ Bradshaw-Rouse
Aug 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I M ShapiroM Pacifici
Oct 21, 1999·Cell Biology International·E GiontiR Porta
May 3, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R T BallockB C Mita
Apr 1, 1992·The Journal of Cell Biology·F Descalzi CanceddaR Cancedda
Jan 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J AlvarezJ M López
Mar 30, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J AlvarezJ M López
Apr 29, 2015·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Vahid Khalilzad-SharghiShadi F Othman
Jan 1, 1997·Connective Tissue Research·J L AriasA I Caplan
Nov 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M IwamotoM Pacifici
Sep 22, 2007·Journal of Cellular Physiology·Sherrill L AdamsLuisa Lassová
Sep 6, 2003·Birth Defects Research. Part C, Embryo Today : Reviews·Andrea D WestonT Michael Underhill
Aug 10, 2011·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Hyuk-Jae KwonHan-Sung Jung
Apr 23, 2015·Journal of Cellular Physiology·Richard T PiszczatowskiNathan H Lents
Aug 17, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K BerggrenC J Forehand
Dec 11, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·U H DietzR Balling
Aug 15, 1994·Microscopy Research and Technique·M IwamotoM Pacifici
Jul 1, 1993·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·P J NeameL C Rosenberg
Nov 26, 2002·The Journal of Biological Chemistry·Wei WangThorsten Kirsch
Mar 18, 2000·The Journal of Biological Chemistry·H EnomotoT Komori

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.