Jul 1, 1992

Type X collagen synthesis in human osteoarthritic cartilage. Indication of chondrocyte hypertrophy

Arthritis and Rheumatism
Klaus von der MarkH Stöss

Abstract

To investigate the appearance of hypertrophic chondrocytes in osteoarthritic (OA) cartilage, using type X collagen as a specific marker. The biosynthesis of type X collagen was examined by metabolic labeling of freshly isolated articular chondrocytes with 3H-proline, immunoprecipitation, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the synthesized collagens. Extracellular deposition of types X and II collagen was analyzed immunohistochemically. Immunostaining revealed an irregular distribution of type X collagen, which was localized around chondrocyte clusters in fibrillated OA cartilage, but was absent from the noncalcified region of normal articular cartilage. Freshly isolated OA chondrocytes synthesized predominantly type X collagen, while control chondrocytes synthesized mostly type II collagen. Our findings indicate focal premature chondrocyte differentiation to hypertrophic cells in OA cartilage.

Mentioned in this Paper

Collagen Type X
Metabolic Process, Cellular
Degenerative Polyarthritis
Extracellular
Chondrocyte Differentiation
Hypertrophic Cardiomyopathy
SDS-PAGE
Structure of Articular Cartilage
Fibrillation
Hypertrophy

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