PMID: 7539146May 23, 1995Paper

Types of Listeria monocytogenes predicted by the positions of EcoRI cleavage sites relative to ribosomal RNA sequences

Proceedings of the National Academy of Sciences of the United States of America
R HubnerJ A Webster

Abstract

By using taxonomic characters derived from EcoRI restriction endonuclease digestion of genomic DNA and hybridization with a labeled rRNA operon from Escherichia coli, a polymorphic structure of Listeria monocytogenes, characterized by fragments with different frequencies of occurrence, was observed. This structure was expanded by creating predicted patterns through a recursive process of observation, expectation, prediction, and assessment of completeness. This process was applied, in turn, to normalized strain patterns, fragment bands, and positions of EcoRI recognition sites relative to rRNA regions. Analysis of 1346 strains provided observed patterns, fragment sizes, and their frequencies of occurrence in the patterns. Fragment size statistics led to the creation of unobserved combinations of bands, predicted pattern types. The observed fragment bands revealed positions of EcoRI sites relative to rRNA sequences. Each EcoRI site had a frequency of occurrence, and unobserved fragment sizes were postulated on the basis of knowing the restriction site locations. The result of the recursion process applied to the components of the strain data was an extended classification with observed and predicted members.

References

Dec 1, 1979·Analytical Biochemistry·E M Southern
Sep 1, 1986·Annales De L'Institut Pasteur. Microbiology·F Grimont, P A Grimont
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T MiyataT Nishida
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·J L BruceJ A Webster

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Citations

Feb 15, 2002·International Journal of Food Microbiology·Maija-Liisa SuihkoPatrick Gustavsson
Aug 10, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·C JacquetJ Rocourt
May 2, 2000·Applied and Environmental Microbiology·M WiedmannK J Boor
Jan 3, 2001·Applied and Environmental Microbiology·D M NortonM Wiedmann
Feb 7, 2001·Applied and Environmental Microbiology·D M NortonM Wiedmann
Oct 7, 2004·Applied and Environmental Microbiology·Michael J GrayMartin Wiedmann
Jun 28, 2001·Journal of Clinical Microbiology·C A NadonM Wiedmann
Jan 5, 2002·Journal of Clinical Microbiology·G BasagliaP De Paoli
Sep 11, 2004·Journal of Applied Microbiology·K SuzukiH Yamashita
Sep 13, 2017·International Journal of Systematic and Evolutionary Microbiology·Yuki AsakawaMasayuki Aizawa
Jan 27, 2004·International Journal of Systematic and Evolutionary Microbiology·Koji SuzukiHiroshi Yamashita
Sep 18, 2002·Journal of Food Protection·W Payton PruettBonnie Rose
Jul 3, 2002·Journal of Food Protection·J S BaileyD E Cosby
Sep 18, 1997·Applied and Environmental Microbiology·E T RyserC W Donnelly

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