Typing of Clostridium difficile isolates endemic in Japan by sequencing of slpA and its application to direct typing

Journal of Medical Microbiology
Haru KatoYoshichika Arakawa

Abstract

A typing system for Clostridium difficile using sequencing of the surface-layer protein A encoding gene (slpA) was evaluated and used to analyse clinical isolates in Japan. A total of 160 stool specimens from symptomatic patients in Japan was examined and 87 C. difficile isolates were recovered. slpA sequence typing was found to have reliable typability and discriminatory power in comparison with PCR ribotyping, and the typing results were highly reproducible and comparable. slpA sequence typing was used to type C. difficile in DNA extracted directly from stool specimens. Among the 90 stool specimens in which direct typing results were obtained, 77 specimens were positive for C. difficile culture, and typing results from isolated strains agreed with those from direct typing in all 77 specimens. The slpA sequence type smz was dominant at all four hospitals examined, and this endemic type was detected by culture and/or direct typing in 61 (62 %) of 99 stool specimens positive for toxic culture and/or direct slpA sequence typing. Comparison of epidemic strains reported throughout the world revealed one isolate identified as slpA sequence type gc8, which was found to correspond to PCR ribotype 027 (BI/NAP1/027), whereas no isolates...Continue Reading

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Citations

Aug 16, 2014·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Satoshi NishimuraShujiro Yazumi
Aug 6, 2015·BMC Microbiology·Darius GawlikStefan Monecke
Feb 24, 2015·Research in Microbiology·Sandra Janezic, Maja Rupnik
Feb 15, 2018·Infectious Diseases and Therapy·Thomas V Riley, Tomomi Kimura
Mar 28, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M N MavrosM E Falagas
Feb 6, 2018·Annals of Laboratory Medicine·Nobuaki Mori, Takashi Takahashi
Feb 16, 2019·Clinical Journal of Gastroenterology·Noriaki OguriTadakazu Hisamatsu
Dec 15, 2020·Journal of Environmental Health Science & Engineering·Akram BaghaniMasoumeh Douraghi

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