Typing of hepatic nonparenchymal cells using fibulin-2 and cytoglobin/STAP as liver fibrogenesis-related markers

Histochemistry and Cell Biology
Yuko TateakiKatsutoshi Yoshizato

Abstract

Fibulin-2 and cytoglobin/stellate cell activation-associated protein (Cygb/STAP) are considered to be markers of hepatic myofibroblasts (MFs) and stellate cells (HSCs), respectively. The aim of the present study was to characterize the nonparenchymal cells (NPCs) of normal rat livers and carbon tetrachloride-induced fibrotic rat livers with respect to the expression of these two proteins. NPCs in normal (Glisson's capsules) and fibrotic (fibrotic septa) connective tissues were immunohistochemically categorized into four cell types in terms of the expression of fibulin-2 and Cygb/STAP: fibulin-2 and Cygb/STAP double-positive (Fib(+)/STAP(+)); fibulin-2-positive and Cygb/STAP-negative (Fib(+)/STAP(-)); Fib(-)/STAP(+); and Fib(-)/STAP(-). The Glisson's capsules had Fib(+)/STAP(+) and Fib(-)/STAP(-) cell occupancy rates of 45.5% and 54.5%, respectively, but did not contain Fib(+)/STAP(-) or Fib(-)/STAP(+) cells. On the other hand, the fibrotic septa contained Fib(+)/STAP(+), Fib(-)/STAP(+), and Fib(-)/STAP(-) cells at occupancy rates of 35.0%, 50.5%, and 9.1%, respectively, but did not contain Fib(+)/STAP(-) cells. Thus, fibrosis is characterized by a dramatic increase in Fib(-)/STAP(+) NPCs, and a dramatic decrease in Fib(-)/STAP(...Continue Reading

Citations

Dec 23, 2004·Histochemistry and Cell Biology·Esther Asan, Detlev Drenckhahn
Nov 12, 2005·Histochemistry and Cell Biology·Douglas J Taatjes, Jürgen Roth
Oct 7, 2006·Histochemistry and Cell Biology·Tomohiro OgawaKatsutoshi Yoshizato
Feb 2, 2012·World Journal of Gastroenterology : WJG·Wen-Ting BinXiao-Lin Shi
Aug 11, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Pradeep P A MammenDaniel J Garry
Jul 12, 2011·Cellular and Molecular Life Sciences : CMLS·Urszula OleksiewiczGeorge Xinarianos
Dec 1, 2015·Frontiers in Physiology·Norifumi Kawada
May 27, 2011·Journal of Pharmacological Sciences·Tomoharu MaruyamaHiroshi Ozaki
Mar 31, 2015·PloS One·Michel FaustherJonathan A Dranoff
Dec 9, 2016·Cytotechnology·Ayşegül DoğanFikrettin Şahin
Feb 18, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jin Seok KangShoji Fukushima
Apr 12, 2008·European Journal of Gastroenterology & Hepatology·Kin Jip CheungHans Van Vlierberghe
Apr 19, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yong-Han PaikDavid A Brenner
May 13, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sankalpa ChakrabortyAlo Nag
Jun 5, 2020·Clinical and Molecular Hepatology·Le Thi Thanh ThuyNorifumi Kawada
Sep 1, 2004·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Paolo AscenziThorsten Burmester

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.