Typing of human and animal strains of influenza virus with conserved signature peptides of matrix M1 protein by high resolution mass spectrometry

Journal of Virological Methods
Alexander B SchwahnK M Downard

Abstract

The use of high resolution mass spectrometry to detect signature peptides within proteolytic digests of the isolated matrix M1 protein, and whole virus digests, for both human and animal strains of influenza is shown to be able to rapidly and reliably type the virus. Conserved sequences for predicted tryptic peptides were identified through alignments of matrix M1 protein sequences across all human, avian and swine strains of the influenza virus. Peptides with unique masses, when compared with those from the in silico digestion of all influenza antigens and those proteins known to contaminate egg grown strains, were identified using the purpose built FluGest algorithm. Their frequency of occurrence within the matrix M1 protein across all type A and type B strains was established with the FluAlign algorithm. The subsequent detection of the signature peptides of matrix M1 protein within proteolytic digests of type A and type B human and avian strains has been demonstrated.

References

Nov 11, 1989·Nucleic Acids Research·L Wakefield, G G Brownlee
Nov 26, 1999·Biochemistry·J G Kiselar, K M Downard
Jun 22, 2000·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·E StarickO Werner
Nov 23, 2000·Journal of Virology·P Gómez-PuertasA Portela
Feb 13, 2004·Bioinformatics·Michele ClampGeoffrey J Barton
Aug 9, 2005·The Veterinary Record·L D SimsP Roeder
Jun 26, 2007·Journal of Virological Methods·Bethny MorrisseyKevin M Downard
Jun 27, 2007·The Analyst·Kevin M Downard, Bethny Morrissey
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Oct 19, 2007·Journal of Virology·Yiming BaoDavid Lipman
Jul 5, 2008·Journal of Virological Methods·Kamol SuwannakarnYong Poovorawan
Sep 12, 2008·Mass Spectrometry Reviews·Kevin M DownardAlexander B Schwahn
May 1, 2009·Analytical Chemistry·Alexander B SchwahnKevin M Downard

❮ Previous
Next ❯

Citations

Aug 16, 2011·Analytica Chimica Acta·Jie ZhengKai Tang
Oct 26, 2010·Journal of Virological Methods·Alexander B Schwahn, Kevin M Downard
Jul 30, 2010·Analytical Biochemistry·Marybeth C CreskeyTerry D Cyr
Jan 22, 2014·Journal of Mass Spectrometry : JMS·Neil D Fernandes, Kevin M Downard
Aug 25, 2015·Frontiers in Microbiology·Neelja SinghalJugsharan S Virdi
Dec 17, 2014·Journal of Virological Methods·Joanna A Majchrzykiewicz-KoehorstArmand Paauw
Dec 14, 2012·Analytical Chemistry·An P Nguyen, Kevin M Downard
Feb 2, 2013·The Analyst·An P Nguyen, Kevin M Downard
Nov 19, 2020·ACS Infectious Diseases·Nicholas L DollmanKevin M Downard

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.