PMID: 8582134Aug 1, 1995Paper

Typing of sequential bacterial isolates by pulsed-field gel electrophoresis

Diagnostic Microbiology and Infectious Disease
A I HartsteinA M LeMonte

Abstract

We typed 39 sets of multiple bacterial isolates of the same species from patients by pulsed-field gel electrophoresis of genomic DNA (PFGE). Isolates were cultured from different sites or over a 2-week or longer interval. Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae were tested. Excluding E. cloacae, 28 of 32 sets of isolates (87%) demonstrated only identical or highly related PFGE types. Four of the seven sets of E. cloacae showed different types. For species other than E. cloacae, our results suggest that patients are usually colonized and infected with a single strain of these bacterial pathogens. Unlike all of the other tested species, E. cloacae PFGE typing differences suggested the presence of multiple strains causing colonization and infection.

References

Mar 1, 1989·The Journal of Infectious Diseases·J J LiPumaO M Korzeniowski
Oct 1, 1993·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·R V Goering
Jun 1, 1994·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P ChetchotisakdA I Hartstein
Feb 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·B OjeniyiN Høiby
Aug 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J N MaslowR D Arbeit
May 8, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A SametB Nowicki

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Citations

Jul 27, 2004·Antimicrobial Agents and Chemotherapy·Paola StefanelliPaola Mastrantonio
Sep 7, 2006·Journal of Clinical Microbiology·Paola StefanelliPaola Mastrantonio
Dec 10, 2003·Journal of Clinical Microbiology·Paola StefanelliPaola Mastrantonio
Aug 25, 2009·BMC Infectious Diseases·Paola StefanelliPaola Mastrantonio
Feb 2, 2010·Emerging Infectious Diseases·Cecilia FazioPaola Stefanelli
Sep 5, 2012·Enfermedades infecciosas y microbiología clínica·Bernadete L LiphausTelma Regina Marques-Pinto-Carvalhanas
Jul 31, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Rogerio Hakio KuboyamaMaria Luiza Moretti-Branchini
Jul 21, 1998·Annals of the New York Academy of Sciences·E P de VilliersB A Allsopp

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