PMID: 8999287Jan 1, 1996Paper

Typing of verotoxins by DNA colony hybridization with poly- and oligonucleotide probes, a bead-enzyme-linked immunosorbent assay, and polymerase chain reaction

Microbiology and Immunology
Shinji YamasakiY Takeda

Abstract

To identify the type of Verotoxins (VT) produced by Verocytotoxin-producing Escherichia coli (VTEC), a sensitive bead-enzyme-linked immunosorbent assay and polymerase chain reaction with common and specific primers to various VTs (VT1, VT2, VT2vha, VT2vhb, and VT2vp1) were developed. Together with colony hybridization tests with oligo- and polynucleotide probes, these methods were applied to VTEC isolates to type the VT produced. The toxin types of 26 of 37 strains were identified, but the reaction profiles in assays of the remaining 11 strains suggested the existence of new VT2 variants. The application of these identification procedures may be useful as a tool for clinical and epidemiological studies of VTEC infection.

References

Jul 1, 1992·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·K HirataY Takeda
Jan 1, 1992·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·T MeyerJ Heesemann
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Feb 1, 1991·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·H TanakaY Takeda
Jan 1, 1988·Microbiology and Immunology·Y OkuY Takeda
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S B CalderwoodJ J Mekalanos

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Citations

Dec 19, 1998·Microbiology and Immunology·H AsakuraK Takeshi
Jan 9, 2003·Microbiology and Immunology·Hiroshi NakaoTae Takeda
Jan 3, 2001·Applied and Environmental Microbiology·H KobayashiK Yamamoto
Jul 4, 2003·Applied and Environmental Microbiology·Simanti DattaG Balakrish Nair
Sep 6, 2003·Applied and Environmental Microbiology·Hideki KobayashiKôsi Yamamoto
May 8, 2003·Journal of Clinical Microbiology·Sadjia BekalJosée Harel
Jun 7, 2003·Journal of Clinical Microbiology·Claudia TomaMasaaki Iwanaga
Jan 22, 2002·Akusherstvo i ginekologii︠a︡·L GŭrchevaA Dimitrov
Aug 28, 2012·Journal of Laboratory Physicians·Veena A ShettyIndrani Karunasagar
Aug 30, 2014·International Journal of Environmental Research and Public Health·Juan Puño-SarmientoGerson Nakazato
Jan 30, 2016·International Journal of Food Microbiology·Rabee A OmbarakShinji Yamasaki
Nov 22, 2002·FEMS Immunology and Medical Microbiology·Hiroshi NakaoTae Takeda
Mar 27, 2004·FEMS Microbiology Letters·H S KumarShinji Yamasaki
Nov 10, 2007·Memórias do Instituto Oswaldo Cruz·Vanessa BuerisLuiz Rachid Trabulsi
Mar 22, 2001·International Journal of Environmental Health Research·U K ChattopadhyayD Pal
May 26, 2012·Japanese Journal of Infectious Diseases·Takayuki KonnoShioko Saito
May 3, 2019·BMC Research Notes·Diana E WaturangiEdita Aliwarga
Mar 20, 2015·The Journal of Veterinary Medical Science·Katsuhiro TogashiWataru Sato
Aug 21, 2019·International Journal of Environmental Research and Public Health·C M N Khabo-Mmekoa, M N B Momba
Apr 17, 2018·Veterinary World·Sreepriya PrakasanBinaya Bhusan Nayak

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