Tyrosinase immobilization on aminated magnetic nanoparticles by physical adsorption combined with covalent crosslinking with improved catalytic activity, reusability and storage stability

Analytica Chimica Acta
Dong-Mei LiuYan-Ping Shi

Abstract

In the present study, the immobilization method of physical adsorption combined with covalent crosslinking was developed to avoid the shortcomings of both the noncovalent and covalent coupling methods. For the first time, tyrosinase (TYR) was immobilized on the surface of aminated magnetic nanoparticles (Fe3O4-NH2) by the developed method. TYR was firstly adsorbed on the surface of Fe3O4-NH2 by electrostatic interaction, and then by covalent crosslinking with glutaraldehyde (GA), TYR was firmly immobilized on the supports. The immobilized TYR showed enhanced pH and temperature endurances at the optimum pH of 7.0 and temperature of 35 °C. TYR reusability was significantly improved after immobilization and it retained 61.4 ± 2.3% of its initial activity after 5 repeated cycles. Immobilized TYR also showed improved storage stability (73.2 ± 1.1% after 30 days of storage at 4 °C). In addition, the immobilized TYR showed a higher biological affinity to substrate owing to the stabilization of TYR in its active conformation by electrostatic interaction prior to covalent crosslinking. Finally, the immobilized TYR was used to screen of enzyme inhibitors from 11 traditional Chinese medicines (TCMs) to validate whether this method can be ...Continue Reading

Citations

Dec 12, 2019·Environmental Technology·Nana LiJianliang Xue
Feb 9, 2019·International Journal of Biological Macromolecules·Changiz Karami, Mohammad Ali Taher
Jun 3, 2021·International Journal of Molecular Sciences·Alexandra Virginia Bounegru, Constantin Apetrei
Jun 13, 2021·Enzyme and Microbial Technology·Chunzhi LiXiongdiao Lan
Jan 1, 2021·Talanta·Julien BiscayLynn Dennany
Aug 7, 2021·Journal of Pharmaceutical and Biomedical Analysis·Isabela Abreu Trindade XimenesMarcela Cristina de Moraes
Sep 13, 2021·Electrophoresis·Jef RozenskiAnn Van Schepdael
Oct 12, 2021·Critical Reviews in Biotechnology·Saadet AlpdağtaşBarış Binay

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