Tyrosine 331 and phenylalanine 334 in Clostridium perfringens alpha-toxin are essential for cytotoxic activity

FEBS Letters
M JepsonR W Titball

Abstract

Differences in the biological properties of the Clostridium perfringens phospholipase C (alpha-toxin) and the C. bifermentans phospholipase C (Cbp) have been attributed to differences in their carboxy-terminal domains. Three residues in the carboxy-terminal domain of alpha-toxin, which have been proposed to play a role in membrane recognition (D269, Y331 and F334), are not conserved in Cbp (Y, L and I respectively). We have characterised D269Y, Y331L and F334I variant forms of alpha-toxin. Variant D269Y had reduced phospholipase C activity towards aggregated egg yolk phospholipid but increased haemolytic and cytotoxic activity. Variants Y331L and F334I showed a reduction in phospholipase C, haemolytic and cytotoxic activities indicating that these substitutions contribute to the reduced haemolytic and cytotoxic activity of Cbp.

References

Apr 25, 1973·Biochimica Et Biophysica Acta·C M ColleyL L van Deenen
Jun 1, 1984·Archives of Biochemistry and Biophysics·E L Krug, C Kent
Jun 1, 1993·Microbiological Reviews·R W Titball
Apr 3, 1996·Biochimica Et Biophysica Acta·M NagahamaJ Sakurai
Jan 1, 1996·Microbiology and Immunology·M Nagahama, J Sakurai
Oct 6, 1997·The EMBO Journal·J HermosoJ C Fontecilla-Camps
Aug 12, 1998·Nature Structural Biology·C E NaylorA K Basak
Sep 12, 1998·The Journal of Biological Chemistry·M Flores-DíazM Thelestam
Apr 9, 1999·Biochemistry·T Hammarberg, O Rådmark
Dec 28, 1999·Journal of Molecular Biology·C E NaylorB Bolgiano

❮ Previous
Next ❯

Citations

Oct 22, 2003·Journal of Molecular Biology·Graeme C ClarkRichard W Titball
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·Marietta Flores-Díaz, Alberto Alape-Girón
Aug 1, 2012·The Journal of Biological Chemistry·Masataka OdaJun Sakurai
Oct 15, 2010·Acta Crystallographica. Section D, Biological Crystallography·S G VachieriA K Basak
Dec 13, 2002·Microbiology and Immunology·Masahiro NagahamaJun Sakurai
Jul 3, 2007·BMC Structural Biology·Andrei L LomizeHenry I Mosberg
Dec 4, 2015·Toxins·Masataka OdaMasahiro Nagahama
May 28, 2005·The Journal of Biological Chemistry·Marietta Flores-DíazMonica Thelestam
May 17, 2006·Anaerobe·Marietta Flores-DíazAlberto Alape-Girón
Oct 29, 2021·FEBS Open Bio·Laura Monturiol-GrossAlberto Alape-Girón

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.