PMID: 6109731Dec 1, 1980Paper

Tyrosine aminotransferase sensitivity to bromodeoxyuridine during restricted intervals of S phase in hepatoma cells

The Journal of Cell Biology
J C O'Brien

Abstract

Synchronized hepatoma tissue culture (HTC) cells, accumulated at the G1/S boundary with aminopterin, were released into S phase with either thymidine or 5-bromodeoxyuridine (BUdR). Tyrosine aminotransferase (TAT) activity was found to be unaffected by BUdR over the initial 3 h of S phase, but then to rapidly decline to a new basal level of 40% of control by 9 h. There was no corresponding response in the activities of alcohol dehydrogenase, malate dehydrogenase, acid phosphatase, and alkaline phosphatase, or in the rate of protein and RNA synthesis. If BUdR incorporation was restricted to limited periods of S phase, TAT was found to be maximally suppressed by incorporation into the initial 40% of the DNA. Incorporation of the analogue into the latter 60% of DNA synthesized during S phase had no effect on TAT. This is the first report that the effect of BUdR on TAT in HTC cells is associated with incorporation of the analog into DNA synthesized during a specific interval of S phase.

References

Oct 15, 1977·Experimental Cell Research·R van WijkW L Geilenkirchen
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S A SchwartzW H Kirsten
Jul 1, 1975·Cell·W Burke, W L Fangman
Jan 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D MartinD Granner
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R H Stellwagen, G M Tomkins
Jun 11, 1963·Biochimica Et Biophysica Acta·C J THORNEN O KAPLAN

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Citations

Mar 1, 1991·The American Journal of Anatomy·R M ShahE J Feeley

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