PMID: 6109760Jan 1, 1981Paper

Tyrosine hydroxylase activation and inactivation by protein phosphorylation conditions

Journal of Neurochemistry
K E VranaR Roskoski

Abstract

Tyrosine hydroxylase, the rate-limiting enzyme in catecholamine biosynthesis, catalyzes the conversion of tyrosine to DOPA, Cyclic AMP-dependent protein phosphorylation conditions alter tyrosine hydroxylase activity in rat striatal homogenates. In agreement with other laboratories, we find that short-term pre-incubation (3 min) of extracts under phosphorylating conditions (Mg . ATP, cAMP) increases enzyme activity two- to tenfold over control as measured during a subsequent 15-min assay. We now report that preincubation under phosphorylating conditions for longer periods (30 min) results in a loss of activity to levels equal to or below that of the control enzyme. Addition of purified bovine brain protein kinase catalytic subunit and Mg . ATP enhances activation and increases the rate of inactivation. To demonstrate that inactivation is not associated with proteolytic degradation or irreversible denaturation, the inactivated form of the enzyme can be reactivated. The protein kinase inhibitor protein decreases the activation process and prevents inactivation of the enzyme to below control values. The sedimentation coefficient is not changed by phosphorylation conditions (S = 8.8 +/- 0.1). Although the apparent Km of the enzyme f...Continue Reading

References

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Citations

Apr 1, 1986·Journal of Neuroscience Methods·R S ShenC W Abell
Jan 1, 1985·Neurochemistry International·M Messripour, J B Clark
Jul 1, 1994·Neurobiology of Aging·J C PorterW Kedzierski
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K A AlbertP Greengard
May 29, 2014·Journal of Neural Transmission·Izel TekinKent E Vrana
Jan 1, 1983·Journal of Neurochemistry·D Parkinson, R R Rando
Sep 1, 1981·Journal of Neurochemistry·A Vitto, A J Mandell
Dec 1, 1983·Archives of Biochemistry and Biophysics·D B Bennett, C J Coscia
Jun 1, 1983·Journal of Neurochemistry·K E Vrana, R Roskoski
Oct 1, 1988·Journal of Neurochemistry·H Wilgus, R Roskoski
Mar 1, 1987·Journal of Neurochemistry·R RoskoskiD B Glass
Dec 1, 1993·European Journal of Biochemistry·R RoskoskiL M Roskoski
Dec 28, 1983·Biochemical and Biophysical Research Communications·D M Kuhn, W Lovenberg

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