Abstract
Receptor for activated C kinase (Rack)-1 is a protein kinase C-interacting protein, and contains a WD repeat but has no enzymatic activity. In addition to protein kinase C, Rack-1 also binds to Src, phospholipase Cgamma, and ras-GTPase-activating proteins. Thus, Rack-1 is thought to function as a scaffold protein that recruits specific signaling elements. In a cytokine signaling cascade, Rack-1 has been reported to interact with the IFN-alphabeta receptor and Stat1. In addition, we show here that Rack-1 associates with a member of Jak, tyrosine kinase 2 (Tyk2). Rack-1 interacts weakly with the kinase domain and interacts strongly with the pseudokinase domain of Tyk2. Rack-1 associates with Tyk2 via two regions, one in the N terminus and one in the middle portion (aa 138-203) of Rack-1. Jak activation causes the phosphorylation of tyrosine 194 on Rack-1. After phosphorylation, Rack-1 is translocated toward the perinuclear region. In addition to functioning as a scaffolding protein, these results raise the possibility that Rack-1 functions as a signaling molecule in cytokine signaling cascades.
References
Jul 24, 1992·Cell·L VelazquezS Pellegrini
Oct 19, 1995·Nature·J N Ihle
Sep 22, 1994·Nature·E J NeerT F Smith
Jun 3, 1994·Science·J E DarnellG R Stark
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·D RonD Mochly-Rosen
Feb 9, 1996·Cell·J N Ihle
Feb 9, 1996·Cell·M A MerazR D Schreiber
Feb 9, 1996·Cell·J E DurbinD E Levy
Jun 20, 1998·Molecular and Cellular Biology·B Y ChangC A Cartwright
May 20, 1998·Cell·S J RodigR D Schreiber
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R GongoraM D Cooper
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E CrozeO Colamonici
Nov 9, 2000·Immunity·M KaraghiosoffM Müller
Nov 9, 2000·Immunity·K ShimodaK Nakayama
Feb 13, 2001·Immunity·C ParkC Schindler
Mar 30, 2001·The Journal of Biological Chemistry·B Y ChangC A Cartwright
Apr 13, 2001·The Journal of Biological Chemistry·A UsachevaO Colamonici
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kazuya ShimodaMine Harada
Oct 26, 2002·Oncogene·Betty Y ChangChristine A Cartwright
Sep 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna UsachevaOscar R Colamonici
Citations
Jul 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Zhimei DuLionel B Ivashkiv
Dec 24, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Béatrice Payelle-Brogard, Sandra Pellegrini
Jan 15, 2015·The Journal of Biological Chemistry·Wencheng LinShijun J Zheng
Mar 16, 2005·Journal of Virology·Toru KubotaAtsushi Kato
Sep 18, 2013·The Journal of Biological Chemistry·Ryuta MuromotoTadashi Matsuda
Dec 31, 2005·Molecular and Cellular Biology·Weizhou ZhangLu-Hai Wang