PMID: 7519400Jul 1, 1994Paper

Tyrosine kinase involvement in IL-1 beta-induced expression of iNOS by beta-cells purified from islets of Langerhans

The American Journal of Physiology
J A CorbettM L McDaniel

Abstract

Nitric oxide is believed to mediate the inhibitory effects of cytokines on glucose-stimulated insulin secretion by both rat and human islets. The aims of this study were 1) to determine the cellular source of the cytokine-inducible isoform of nitric oxide synthase (iNOS) expressed in islets following cytokine stimulation and 2) to determine whether tyrosine kinase activity participates in cytokine-induced iNOS expression. In this report we demonstrate that the cytokine interleukin-1 beta (IL-1 beta) stimulates the expression of iNOS and the formation of nitric oxide (as determined by nitrite formation, a stable oxidative product of nitric oxide) by isolated intact rat islets and by primary beta-cells purified by fluorescence-activated cell sorting (FACS). Both the expression of iNOS and nitrite formation induced by IL-1 beta were prevented by the mRNA transcriptional inhibitor actinomycin D. IL-1 beta did not induce the expression of iNOS by FACS-purified alpha-cells, the other major endocrine cell type of the islet. The tyrosine kinase inhibitors genistein and herbimycin A prevented IL-1 beta-induced expression of immunoprecipitable iNOS and nitrite release by islets, by insulinoma RINm5F cells, and by FACS-purified beta-cells...Continue Reading

References

Jun 1, 1992·European Journal of Immunology·E MuñozB T Huber
Dec 14, 1990·Biochemical and Biophysical Research Communications·D W Coyne, A R Morrison
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C H JuneL E Samelson
Sep 15, 1989·Biochemical and Biophysical Research Communications·Y UeharaS Mizuno
Sep 1, 1985·Endocrinology·D G PipeleersW Gepts
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Mar 16, 1993·European Journal of Pharmacology·T P MiskoM G Currie
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J A CorbettE R Unanue
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J A CorbettM L McDaniel
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J A CorbettM L McDaniel

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Citations

Jan 5, 1996·The Journal of Biological Chemistry·L C DenlingerJ A Corbett
May 20, 1999·Annual Review of Pharmacology and Toxicology·A J HobbsS Moncada
Aug 28, 2003·Clinical and Experimental Pharmacology & Physiology·Francis I Achike, Chiu-Yin Kwan
Oct 6, 2017·Antioxidants & Redox Signaling·Bryndon J Oleson, John A Corbett
Sep 11, 2019·Nature Reviews. Immunology·Marc Y DonathThomas Mandrup-Poulsen
Oct 24, 1998·Clinical and Experimental Pharmacology & Physiology·R LétienneC Barrès
Aug 17, 2011·International Journal of Molecular Sciences·Yong JiSong Wu
Sep 1, 1996·Diabetologia·T Mandrup-Poulsen
Sep 10, 2009·American Journal of Physiology. Endocrinology and Metabolism·Katherine J HughesJohn A Corbett
Jun 16, 2001·Hypertension·R P CarsonD Robertson
Jan 5, 2021·Frontiers in Immunology·Katerina PyrillouMurray C H Clarke
Nov 1, 1995·Research in Immunology·K FehselV Kolb-Bachofen
Jan 1, 1996·Life Sciences·J L Bankers-FulbrightD J McKean
Dec 31, 1997·Biochimica Et Biophysica Acta·A L ScarimJ A Corbett
Sep 21, 2007·Molecular and Cellular Endocrinology·Eun-Kyung KimByung-Hyun Park
Jul 10, 2020·The Journal of Physical Chemistry. a·Daniel F CoimbraGiovanni F Caramori

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