PMID: 4084552Nov 5, 1985

Tyrosine motions in relation to the ferric spin equilibrium of cytochrome P-450cam

Biochemistry
M T Fisher, Stephen G Sligar

Abstract

Second derivative spectroscopy was used to determine the percentage of tyrosine residues that are exposed to solvent in cytochrome P-450cam isolated from Pseudomonas putida. The ratio between two peak to trough second derivative absorbance differences has been shown to be dependent on the polarity of the microenvironment surrounding tyrosine residues [Ragone, R., Colonana, G., Balestrieri, C., Servillo, L., & Irace, G. (1984) Biochemistry 23, 1871]. With a number of camphor analogues that independently vary the spin equilibrium of the ferric cytochrome P-450 cam, experiments have demonstrated that the percentage of tyrosine residues exposed to solvent is linearly dependent on the percentage of ferric high-spin species present. This is not simply a function of the extent of substrate binding since in all cases the substrate concentration was sufficient to ensure saturation of the cytochrome. The local microenvironment of approximately one tyrosine residue appears to be linearly correlated with the percentage of ferric high-spin cytochrome. Structural studies of cytochrome P-450cam using small-angle X-ray scattering [Lewis, B. A., & Sligar, S. G. (1983) J. Biol. Chem. 258, 3599] and high-pressure difference spectroscopy [Fisher, ...Continue Reading

References

Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Stephen G Sligar, I C Gunsalus
Jul 1, 1985·Archives of Biochemistry and Biophysics·M T FisherStephen G Sligar
Aug 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·R TsaiH Beinert
Feb 29, 1972·Biochemistry·E MünckI C Gunsalus
Nov 6, 1974·Biochemical and Biophysical Research Communications·Stephen G SligarI C Gunsalus
Feb 1, 1984·Archives of Biochemistry and Biophysics·R E WhiteStephen G Sligar
Jan 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·A S Brill, H E Sandberg

Citations

Jan 27, 2004·Biochemical and Biophysical Research Communications·Nikitas BistolasUlla Wollenberger
May 2, 2002·Biochimica Et Biophysica Acta·R Lange, Claude Balny
May 2, 2002·Biochimica Et Biophysica Acta·G Hui Bon HoaStephen G Sligar
Sep 18, 2001·European Journal of Biochemistry·P Hlavica, D F Lewis
Feb 15, 1988·European Journal of Biochemistry·R LangeA Bienvenüe
Oct 15, 1992·European Journal of Biochemistry·C Di PrimoStephen G Sligar
Jan 15, 1998·European Journal of Biochemistry·F BancelR Lange
Jun 11, 2004·Annual Review of Biochemistry·Olena Pylypenko, Ilme Schlichting
Jan 20, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Alicja FrankeRudi van Eldik
Jan 1, 1993·Drug Metabolism Reviews·L KoymansN P Vermeulen
Jan 21, 2014·Journal of Raman Spectroscopy : JRS·Piotr J MakJames R Kincaid
Jul 1, 1991·Archives of Biochemistry and Biophysics·M SchweizerM Oertle
Jul 28, 1987·Biochemistry·M T Fisher, Stephen G Sligar
Jul 26, 2007·The Journal of Biological Chemistry·Ilia G DenisovStephen G Sligar
Jun 12, 2020·Soft Matter·Tyler Camp, Stephen G Sligar

Related Concepts

Cytochrome P-450 Oxygenase
Heme
Oxidation-Reduction
Protein Conformation
Protein Denaturation
Pseudomonas
Spectrophotometry
Tyrosine

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.