PMID: 8972730Nov 8, 1996Paper

Tyrosine phosphorylation and translocation of phospholipase C-gamma 2 in polymorphonuclear leukocytes treated with pervanadate

Biochimica Et Biophysica Acta
N KawakamiS Fujimoto

Abstract

We examined in detail the tyrosine phosphorylation of proteins, especially inositol phospholipid-specific phospholipase C (PLC) gamma 2, during activation of respiratory burst of guinea pig polymorphonuclear leukocytes (PMNs) by pervanadate. The pervanadate, generated from a combination of H2O2 and orthovanadate, induced concomitantly tyrosine phosphorylation of 145, 120, 104, 76, 68, 60, 53, 42, 37, 28, and 25 kDa proteins and superoxide anion (O2-) production of PMNs. The pretreatment of PMNs with genistein caused an inhibition of tyrosine phosphorylation of these proteins, and also markedly depressed O2- production. Among the above proteins, a 145 kDa protein was found to be identical with the protein recognized by the anti-PLC gamma 2 antibody on Western blots. PLC gamma 2 was detected in the cytosol fraction but not in the membrane fraction of resting PMNs, whereas it was detected in both cytosol and membrane fractions of pervanadate treated PMNs. PLC activity of pervanadate treated PMNs was higher than that of resting cells. In addition, the enzyme activity of the cytosol fraction from the former cells was significantly lower than that from the latter cells, whereas the enzyme activity of membrane fraction from the former...Continue Reading

References

Mar 23, 1978·The New England Journal of Medicine·B M Babior
Nov 1, 1975·The Journal of Clinical Investigation·I M GoldsteinG Weissmann
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M I WahlG Carpenter
Jun 28, 1985·Biochemical and Biophysical Research Communications·P M LadI S Grewal

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Citations

Jun 9, 2015·International Journal of Molecular Sciences·Jan KorbeckiDariusz Chlubek
Apr 28, 2004·Free Radical Biology & Medicine·Masayuki NiwaSadaki Fujimoto
Dec 14, 2017·Journal of Cellular Biochemistry·Xiaoguang ChenJun Ma
Jun 11, 1999·The American Journal of Physiology·J G GarciaV Natarajan
Dec 25, 2003·Journal of Enzyme Inhibition and Medicinal Chemistry·Ana Claudia Galvão FreireCarmen Veríssima Ferreira
Jan 24, 1998·Archives of Biochemistry and Biophysics·N KawakamiS Fujimoto

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