Tyrosine phosphorylation generates multiple isoforms of the mitochondrial capsule protein, phospholipid hydroperoxide glutathione peroxidase (PHGPx), during hamster sperm capacitation

Biology of Reproduction
Subir K NagdasGary E Olson

Abstract

Sperm capacitation is a maturation process, occurring in the female reproductive tract, that produces fertilization-competent spermatozoa. Protein tyrosine phosphorylation represents an important event in capacitation. The present study demonstrates the capacitation-dependent tyrosine-phosphorylation of phospholipid hydroperoxide glutathione peroxidase (PHGPx), the disulfide cross-linked, major structural protein of the sperm mitochondrial capsule. Immunofluorescence microscopy using an antiphosphotyrosine monoclonal antibody (anti-pY20) demonstrated the presence of capacitation-associated tyrosine phosphorylated proteins in the flagellum of hamster spermatozoa. Among the tyrosine-phosphorylated polypeptides (M(r) 19,000- 99,000), a 19-kDa polypeptide was the only one that can be solubilized completely by Triton X-100-dithiothreitol (DTT). The 19-kDa polypeptide was purified by anion-exchange chromatography and by immunoaffinity chromatography. Proteomic identification of the 19-kDa polypeptide by nano-electrospray tandem mass spectrometry yielded six peptides that matched the National Center for Biotechnology Information (NCBI) database sequences of bovine PHGPx. Indirect immunofluorescence localized PHGPx to the midpiece of t...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1988·Gamete Research·J Hiraoka, Y Hirao
Sep 1, 1987·Journal of Reproduction and Fertility·H I CalvinS I Turner
Feb 1, 1985·Biology of Reproduction·K J Go, D P Wolf
Oct 1, 1970·Journal of Reproduction and Fertility·R Yanagimachi
Dec 10, 1982·The Journal of Experimental Zoology·E Ohzu, R Yanagimachi
Nov 1, 1994·The American Journal of Physiology·A Cohen-Dayag, M Eisenbach
Jan 1, 1996·Biology of Reproduction·S K NagDasG E Olson
Feb 21, 1997·The Journal of Biological Chemistry·S VijayaraghavanD W Carr
Jul 23, 1998·Biology of Reproduction·P E Visconti, G S Kopf
Jul 23, 1998·Biology of Reproduction·N L Cross
Oct 15, 1998·Molecular Reproduction and Development·P KalabJ Moos
Sep 25, 2001·BioFactors·A RoveriM Maiorino
Dec 4, 2001·Journal of Reproductive Immunology·P E ViscontiA B Diekman
Mar 20, 2002·Methods in Enzymology·Antonella RoveriFulvio Ursini
Aug 24, 2002·Biology of Reproduction·Carlo ForestaMatilde Maiorino
Aug 23, 1952·Nature·C R AUSTIN

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Citations

Apr 28, 2005·Free Radical Biology & Medicine·Mauro SalviAntonio Toninello
Aug 2, 2008·Reproduction, Fertility, and Development·Carmen ColasTeresa Muiño-Blanco
Oct 17, 2007·Biological Chemistry·Leopold Flohé
May 9, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Shagufta A KhanVrinda V Khole
Dec 21, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Xiaoli ChenDong Wang
Nov 5, 2013·Molecular and Cellular Biochemistry·Subir K NagdasSamir Raychoudhury
Dec 24, 2013·International Journal of Endocrinology·Md Saidur RahmanMyung-Geol Pang
Oct 28, 2009·Journal of Reproductive Immunology·S ShivajiArchana B Siva
May 8, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·E Arenas-RíosA Rosado
Jul 6, 2010·Molecular Reproduction and Development·Venkatesh KotaSisinthy Shivaji
Jan 16, 2009·Reproduction in Domestic Animals = Zuchthygiene·F J PeñaB Macías García
Jul 12, 2013·Biology of Reproduction·Laurelle SaccaryFrederick W K Kan
Oct 6, 2009·Molecular and Cellular Biochemistry·Madhumouli ChatterjeeParimal C Sen
Jun 9, 2020·Journal of Proteome Research·Kerekoppa P RameshaThottethodi Subrahmanya Keshava Prasad

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Methods Mentioned

BETA
phase-contrast
immunoprecipitation
ion-exchange chromatography

Software Mentioned

NetPhos
Blast

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