Tyrosine phosphorylation in Escherichia coli

Journal of Molecular Biology
P FreestoneV Norris

Abstract

The phosphorylation on tyrosine of a protein in Escherichia coli both in vivo and in vitro was revealed by recognition by anti-phosphotyrosine antibodies, labelling with [gamma-32P]ATP, and phosphoamino acid analysis. This protein, which we name TypA, is the product of the o591 reading frame as revealed by N-terminal sequencing and antibody cross-reactivity. Inactivation of typA altered the patterns of protein synthesis during both exponential growth and carbon starvation. These alterations included the disappearance of an acidic isoform of both the universal stress protein UspA and carbon starvation protein Csp15, and increased synthesis of the histone-like protein H-NS. The sequence of TypA from strain K-12 differs from that of an enteropathogenic strain in six amino acid residues and the protein is three residues shorter. We propose that TypA interacts with global regulatory networks and that its phosphorylation may be relevant to pathogenesis.

References

Jul 1, 1992·Journal of Bacteriology·M AtkinsonL Sequeira
Sep 1, 1990·Journal of Bacteriology·K Kelly-WintenbergT C Montie
Dec 1, 1991·Molecular Microbiology·V NorrisK L Leach
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J S McCarty, G C Walker
Jan 1, 1991·Methods in Enzymology·J C CortayA J Cozzone
May 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M GassmannU Hübscher
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R A VanBogelen, F C Neidhardt
Sep 1, 1986·European Journal of Biochemistry·J C CortayA J Cozzone
Jan 1, 1983·Methods in Enzymology·J A CooperT Hunter
Jan 1, 1983·Journal of Pediatric Gastroenterology and Nutrition·M M BaldiniH W Moon
Jun 1, 1994·Molecular Microbiology·S L SouthT C Montie
Feb 1, 1995·Molecular Microbiology·P FreestoneV Norris
Mar 24, 1995·Science·A Levitzki, A Gazit
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M Y Sherman, A L Goldberg
Jan 1, 1993·Journal of Cellular Biochemistry
Jan 1, 1993·Journal of Cellular Biochemistry·A J Cozzone
Aug 1, 1995·Molecular Microbiology·S Y QiC D O'Connor
Jun 28, 1996·Journal of Molecular Biology·B DuclosA J Cozzone
Aug 1, 1996·Journal of Bacteriology·P J Kennelly, M Potts
Apr 1, 1997·Journal of Bacteriology·B McCartneyM Potts
Apr 1, 1997·Journal of Bacteriology·S C SmithM Potts

❮ Previous
Next ❯

Citations

Jul 15, 2003·Clinical Microbiology Reviews·S C ClarkeP H Williams
Oct 14, 2008·BMC Genomics·Chantal W NdeWilliam E Bentley
Jul 28, 2007·BMC Infectious Diseases·Dennis J Murphy, James R Brown
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J WuA Newton
Aug 4, 2006·Journal of Plant Physiology·Min-Xia ChouJun-Chu Zhou
Nov 4, 2006·Microbial Pathogenesis·Wen-Tssann LiuC M Anjam Khan
Jan 7, 2004·Biotechnology and Bioengineering·Pat S Lee, Kelvin H Lee
Mar 18, 2015·Journal of Bacteriology·Promisree Choudhury, Ann M Flower
Sep 25, 2019·Journal of Bacteriology·Spencer HavisSteven J Bark
Jan 12, 1999·Microbiology·P FreestoneV Norris
Jul 28, 2020·Journal of Molecular Biology·Michelle R GibbsKurt Fredrick
Jul 9, 1999·Progress in Lipid Research·C C DiRussoJ D Weimar
May 7, 2003·Current Opinion in Microbiology·Kristian KvintThomas Nyström
Aug 2, 2003·Research in Microbiology·Ronan O'Toole, Huw D Williams
Jul 29, 2006·Mutation Research·Yixin Yang, Douglas Fix
Feb 8, 2006·Biochemistry·Arnim Weber, Kirsten Jung

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.