Tyrosine phosphorylation of alpha tubulin in human T lymphocytes

European Journal of Immunology
S C LeyM J Crumpton

Abstract

N-terminal sequencing of the 55- and 50-kDa polypeptides affinity purified on a phosphotyrosine monoclonal antibody column from activated Jurkat T cells identified alpha and beta tubulin. Two-dimensional gel analysis indicated that alpha tubulin was directly phosphorylated on tyrosine. beta Tubulin was not detectably tyrosine phosphorylated but was precipitated by anti-phosphotyrosine (PTyr) antibody by virtue of its association with the alpha subunit as a heterodimer. Phosphotyrosyl alpha tubulin was not incorporated into intact microtubules and was all in the unpolymerized soluble fraction. These results suggest that tyrosine phosphorylation of alpha tubulin may inhibit the ability of this subunit to polymerize into microtubules. Stimulation of Jurkat T cells via T cell receptor increased the amount of tubulin precipitated by the anti-PTyr antibody. These data raise the possibility that the polymerization of tubulin heterodimers may be regulated by phosphorylation on tyrosine during T cell activation.

References

Feb 14, 1992·Science·E EttehadiehR Aebersold
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A A DaviesM J Crumpton
Jan 1, 1991·Annual Review of Cell Biology·B M Sefton, M A Campbell
Nov 1, 1990·The Journal of Cell Biology·W T MattenP F Maness
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A WeissT Kadlecek
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D J ParkS G Rhee
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C H JuneL E Samelson
Feb 1, 1989·Current Opinion in Cell Biology·E Mandelkow, E M Mandelkow
Jan 1, 1988·Annual Review of Cell Biology·K F Sullivan
Jan 1, 1988·Annual Review of Immunology·H CleversC Terhorst
Nov 1, 1981·The Journal of Experimental Medicine·R J RobbK A Smith
Jan 1, 1982·Methods in Cell Biology·W C Thompson

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Citations

Sep 19, 2008·Planta·Yaroslav BlumePavel Dráber
Jul 25, 2003·Experimental Cell Research·Graziella CappellettiRosalba Maci
Jan 1, 1996·The Journal of Cell Biology·M YasunagaS Nishikawa
Sep 19, 2012·The Journal of Cell Biology·Laura Andrés-DelgadoMiguel A Alonso
Oct 15, 2010·Journal of Cellular Physiology·Navin K VermaYuri Volkov
Jul 28, 2009·Toxicology and Applied Pharmacology·Hasmik GrigoryanOksana Lockridge
Sep 1, 1996·Immunity·R L Wange, L E Samelson
Oct 27, 2017·International Journal of Molecular Sciences·Dorota WlogaHanna Fabczak
Oct 6, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Amrita RamkumarKassandra M Ori-McKenney
Mar 1, 1997·European Journal of Biochemistry·T H MacRae
Feb 10, 2009·Journal of Neurochemistry·Lorelei B Silverman-Gavrila, Milton P Charlton
Feb 25, 2018·Scientific Reports·O G ZatsepinaA A Makarov
Apr 6, 2006·Journal of Receptor and Signal Transduction Research·Jens GoebelThomas L Roszman
Jan 1, 1997·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·N B LaferrièreD L Brown
Jun 20, 1998·The Journal of Biological Chemistry·R D HubyS C Ley
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J A EplerY Shimizu

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