PMID: 8973551Dec 1, 1996Paper

Tyrosine phosphorylation of band 3 protein in Ca2+/A23187-treated human erythrocytes

The Biochemical Journal
Giampaolo MinettiA Brovelli

Abstract

Human erythrocytes were induced to release membrane vesicles by treatment with Ca2+ and ionophore A23187. In addition to the biochemical changes already known to accompany loading of human erythrocytes with Ca2+, the present study reveals that tyrosine phosphorylation of the anion exchanger band 3 protein also occurs. The relationship between tyrosine phosphorylation of band 3 and membrane vesiculation was analysed using quinine (a non-specific inhibitor of the Ca(2+)-activated K+ channel, and the only known inhibitor of Ca(2+)-induced vesiculation) and charybdotoxin, a specific inhibitor of the apamin-insensitive K(+)-channel. Both inhibitors suppressed tyrosine phosphorylation of band 3. In the presence of quinine, membrane vesiculation was also suppressed. In contrast, at the concentration of charybdotoxin required to suppress tyrosine phosphorylation of band 3, membrane vesiculation was only mildly inhibited (16-23% inhibition), suggesting that tyrosine phosphorylation of band 3 is not necessary for membrane vesiculation. Phosphorylation of band 3 was in fact observed when erythrocytes were induced to shrink in a Ca(2+)-independent manner, e.g. by treatment with the K+ ionophore valinomycin or with hypertonic solutions. The...Continue Reading

Citations

May 12, 2010·European Journal of Clinical Investigation·Michael FöllerFlorian Lang
Jun 4, 2018·Acta Biochimica Et Biophysica Sinica·Yajin ZhaoMan Hou
Apr 4, 2007·Annals of the New York Academy of Sciences·Marcantonio BragadinLuciana Bordin
Dec 13, 2005·American Journal of Physiology. Cell Physiology·Barbara A KlarlFlorian Lang
Feb 8, 2007·Pflügers Archiv : European journal of physiology·Olivier M NiemoellerFlorian Lang
Nov 3, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Peter J Bentzen, Florian Lang
Feb 21, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Peter J BentzenFlorian Lang
Jan 23, 2014·Critical Care Medicine·Takashi MuroyaGeorge C Tsokos
Jul 30, 2016·Frontiers in Physiology·Asya MakhroLars Kaestner
Apr 20, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Philipp A LangFlorian Lang
Jan 25, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Nadezhda N BarvitenkoRoy E Weber
Jun 14, 2000·Animal Reproduction Science·E Töpfer-PetersenM Ekhlasi-Hundrieser
Nov 24, 2004·Biochemical and Biophysical Research Communications·Roman HudecL'udovít Varecka
Aug 9, 2005·Biochimica Et Biophysica Acta·Luciana BordinPhilip S Low
Mar 27, 2007·Biochimica Et Biophysica Acta·Michael R CondonZoltan Spolarics
Sep 10, 2008·Archives of Biochemistry and Biophysics·Cristina FioreGiulio Clari
Nov 26, 2009·Journal of Proteomics·Niels LionJean-Daniel Tissot

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