Tyrosine phosphorylation of GSalpha and inhibition of bradykinin-induced activation of the cyclic AMP pathway in A431 cells by epidermal growth factor receptor.

The Journal of Biological Chemistry
C LiebmannF D Boehmer

Abstract

An increasing amount of experimental data suggest that cross-talk exists between pathways involving tyrosine kinases and heterotrimeric G proteins. In a previous study, we demonstrated that bradykinin (BK) increases the intracellular accumulation of cAMP in the human epidermoid carcinoma cell line A431 by stimulating adenylate cyclase activity via a stimulatory G protein (Gsalpha) (Liebmann, C., Graness, A., Ludwig, B., Adomeit, A., Boehmer, A., Boehmer, F.-D., Nürnberg, B., and Wetzker, R. (1996) Biochem. J. 313, 109-118). Here, we present several lines of evidence indicating the ability of epidermal growth factor (EGF) to suppress BK-induced activation of the cAMP pathway in A431 cells via tyrosine phosphorylation of Gsalpha. Gsalpha was specifically immunoprecipitated from A431 cells using the anti-alphas antiserum AS 348. Tyrosine phosphorylation of Gsalpha was detectable in EGF-pretreated cells with monoclonal anti-phosphotyrosine antibodies. Additionally, A431 cells were labeled with [32P]orthophosphate in vivo and treated with EGF, and the resolved immunoprecipitates were subjected to amino acid analysis. The results clearly indicate that EGF induces tyrosine phosphorylation of Gsalpha in A431 cells. Treatment of A431 ce...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·W P HausdorffR J Lefkowitz
May 25, 1991·European Journal of Pharmacology·C LiebmannK H Jakobs
Jan 15, 1995·The Biochemical Journal·J S MoyersS J Parsons
Aug 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S Rens-Domiano, H E Hamm
Nov 12, 1993·Science·S J Cook, F McCormick
Jun 1, 1994·The Biochemical Journal·B NürnbergG Schultz
Jun 1, 1994·Trends in Biochemical Sciences·K J Blumer, G L Johnson
Feb 15, 1994·Biochemical and Biophysical Research Communications·K SpicherG Schultz
Mar 22, 1996·The Journal of Biological Chemistry·H PoppletonT B Patel

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