Tyrosine phosphorylation of the a kinase anchoring protein 3 (AKAP3) and soluble adenylate cyclase are involved in the increase of human sperm motility by bicarbonate

Biology of Reproduction
M LuconiE Baldi

Abstract

Mammalian testicular spermatozoa are immotile, thus, to reach the oocyte, they need to acquire swimming ability under the control of different factors acting during the sperm transit through the epididymis and the female genital tract. Although bicarbonate is known to physiologically increase motility by stimulating soluble adenylate cyclase (sAC) activity of mammalian spermatozoa, no extensive studies in human sperm have been performed yet to elucidate the additional molecular mechanisms involved. In this light, we investigated the effect of in vitro addition of bicarbonate to human spermatozoa on the main intracellular signaling pathways involved in regulation of motility, namely, intracellular cAMP production and protein tyrosine phosphorylation. Bicarbonate effects were compared with those of the phosphatidyl-inositol-3 kinase inhibitor, LY294002, previously demonstrated to be a pharmacological stimulus for sperm motility. Bicarbonate addition to spermatozoa results in a significant increase in sperm motility as well as in several hyperactivation parameters. This stimulatory effect of bicarbonate and LY294002 is mediated by an increase in cAMP production and tyrosine phosphorylation of the A kinase anchoring protein, AKAP3....Continue Reading

References

Aug 10, 1992·Biochimica Et Biophysica Acta·B Spira, H Breitbart
Apr 1, 1992·The American Journal of Physiology·Z I Cabantchik, R Greger
Jun 1, 1995·Biology of Reproduction·P Leclerc, G S Kopf
Feb 21, 1997·The Journal of Biological Chemistry·S VijayaraghavanD W Carr
Sep 2, 1999·Biochemical and Biophysical Research Communications·F M FleschB M Gadella
Jan 5, 2000·Molecular Reproduction and Development·R A Harrison, N G Miller
Mar 29, 2000·Molecular Reproduction and Development·M L SinclairL R Levin
Aug 31, 2001·Human Reproduction·M LuconiE Baldi
Sep 26, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·H C Ho, S S Suarez
Nov 10, 2001·Trends in Endocrinology and Metabolism : TEM·S B Moss, G L Gerton
Dec 4, 2001·Journal of Reproductive Immunology·P E ViscontiA B Diekman
Aug 9, 2002·Developmental Biology·Kiyoshi MikiEdward M Eddy
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jonathan H ZippinJochen Buck
Dec 24, 2002·The Journal of Biological Chemistry·Ignacio A DemarcoPablo E Visconti
Mar 1, 2003·The Journal of Biological Chemistry·Tatiana N LitvinLonny R Levin
Mar 26, 2003·Reproduction in Domestic Animals = Zuchthygiene·R A P Harrison
Jul 31, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·M Bajpai, G F Doncel
Sep 2, 2003·Molecular Reproduction and Development·Rhona W Baxendale, Lynn R Fraser
Sep 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bijay S Jaiswal, Marco Conti
Sep 27, 2003·The Journal of Biological Chemistry·Nuria Pastor-SolerSylvie Breton
Sep 30, 2003·Nature Cell Biology·Xiao Fei WangHsiao Chang Chan
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Juan F MedinaRonald P J Oude Elferink
Jan 14, 2004·International Journal of Andrology·S S du PlessisM Luconi
Feb 11, 2004·The Journal of Cell Biology·Jonathan H ZippinJochen Buck
Feb 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gloria EspositoByjay S Jaiswal

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Citations

Jan 18, 2011·Asian Journal of Andrology·Yan-Feng LiJohn C Herr
Sep 25, 2012·Asian Journal of Andrology·Debby IckowiczHaim Breitbart
Apr 2, 2009·Nature Reviews. Drug Discovery·Sandra PierreKlaus Scholich
Aug 30, 2008·Human Reproduction·Gabriele VaranoMichaela Luconi
Jul 26, 2008·Human Reproduction Update·Susan S Suarez
May 21, 2008·Molecular Human Reproduction·A BarbonettiS Francavilla
Sep 24, 2005·Biology of Reproduction·Malini BajpaiDaniel W Carr
Nov 13, 2004·Reproductive Biology and Endocrinology : RB&E·Rajesh K Naz, Preeti B Rajesh
Jan 28, 2010·The Journal of Reproduction and Development·Shunsuke TateHiroshi Harayama
Jun 1, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Mariano G BuffoneGustavo F Doncel
Aug 15, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·S MarchianiE Baldi
Apr 19, 2005·Expert Opinion on Biological Therapy·Anil Suri
Aug 5, 2015·Cell and Tissue Research·Mark A Baker
May 26, 2007·Biochemical and Biophysical Research Communications·Carmen BeltránAlberto Darszon
Feb 27, 2008·International Journal of Andrology·Monica MuratoriElisabetta Baldi
Nov 3, 2010·International Journal of Andrology·S MarchianiM Muratori
Aug 23, 2012·Journal of Cellular Physiology·Catherine LachancePierre Leclerc
Mar 24, 2016·ELife·Shatanik MukherjeeDagmar Wachten
Mar 15, 2006·Biochemical and Biophysical Research Communications·Paloma del Carmen Neri-VidaurriMarco T González-Martínez
Mar 15, 2018·Systems Biology in Reproductive Medicine·Sellappan SelvarajuStephen A Krawetz
May 10, 2017·Omics : a Journal of Integrative Biology·Rahim Dad Brohi, Li-Jun Huo
Oct 11, 2007·Molecular Reproduction and Development·Louis-Jean Bordeleau, Pierre Leclerc
Apr 20, 2007·Biology of Reproduction·Travis M HagedornBruce D Schultz

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Methods Mentioned

BETA
electrophoresis
immunoprecipitation
pulls down

Software Mentioned

CASA

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