PMID: 8944643Nov 1, 1996Paper

Tyrosine phosphorylation of the dense plaque protein paxillin is regulated during smooth muscle contraction

The American Journal of Physiology
Z WangS J Gunst

Abstract

Regulation of the attachment of actin filaments to the cell membrane at membrane-associated dense plaque (MADP) sites could allow smooth muscle cells to modulate their cytostructure in response to changes in external stress. In this study, changes in the tyrosine phosphorylation of the MADP protein paxillin were measured by Western blot during the contraction and relaxation of tracheal smooth muscle strips. Tyrosine phosphorylation of paxillin increased by three- to fourfold with a time course similar to force development during contractile stimulation with acetylcholine (ACh), 5-hydroxytryptamine, and KCl and decreased during washout of contractile stimuli and during relaxation induced by forskolin. Immunoprecipitation of muscle extracts with multiple rounds of anti-phosphotyrosine antibody removed approximately 20% of the total paxillin in resting muscles and approximately 60% of paxillin in muscles maximally stimulated with ACh. These results provide the first evidence associating the tyrosine phosphorylation of paxillin with the active contraction of smooth muscle or with any functional response of a fully differentiated tissue in vivo. The results are consistent with a role for MADP proteins in the regulation of force deve...Continue Reading

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Citations

Sep 18, 1997·Journal of Applied Physiology·X ShenS J Gunst
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Luke J Janssen
Jul 18, 2002·The Journal of Physiology·Dale D TangSusan J Gunst
Apr 30, 2003·The Journal of Comparative Neurology·Carl Joakim EkNorman Ruthven Saunders
Mar 16, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·H M Omar FarouqueIan T Meredith
May 16, 2006·Journal of the Society for Gynecologic Investigation·Grazyna B SadowskaBarbara S Stonestreet
Jan 24, 2015·Canadian Journal of Physiology and Pharmacology·Bo LanLinhong Deng
Jul 27, 2017·Scientific Reports·Miguel A ZarateCharles E Wood
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Barbara TolloczkoJames G Martin
Aug 21, 2004·Pediatric Research·Katherine H PeterssonBarbara S Stonestreet
May 10, 2003·Circulation Research·Xueren WangChun Jiang
Jun 29, 2007·American Journal of Physiology. Cell Physiology·Christopher M RemboldShaojie Han
Apr 8, 2009·The Journal of Biological Chemistry·Hai-Lei DingKristine E Kamm
Apr 21, 2010·Experimental Biology and Medicine·Gregory M Dick, Johnathan D Tune
May 20, 2014·The Journal of Physiology·Ming-Ho TsaiJames T Stull
Jun 18, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Grazyna B SadowskaBarbara S Stonestreet
Jul 27, 2000·American Journal of Physiology. Cell Physiology·D MehtaS J Gunst
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Dec 23, 2014·Canadian Journal of Physiology and Pharmacology·Wenwu ZhangSusan J Gunst
Jun 20, 2019·Journal of Muscle Research and Cell Motility·Brian SitThomas Iskratsch
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Sep 1, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Barbara S StonestreetClifford S Patlak
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Apr 15, 2014·Experimental Biology and Medicine·Mariya S SpasovaBarbara S Stonestreet
Feb 13, 2001·Journal of Neuroscience Research·S Peña De Ortiz, Y Arshavsky

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