Tyrosine phosphorylation of the UDP-glucose dehydrogenase of Escherichia coli is at the crossroads of colanic acid synthesis and polymyxin resistance.

PloS One
Soline LacourChristophe Grangeasse

Abstract

In recent years, an idiosyncratic new class of bacterial enzymes, named BY-kinases, has been shown to catalyze protein-tyrosine phosphorylation. These enzymes share no structural and functional similarities with their eukaryotic counterparts and, to date, only few substrates of BY-kinases have been characterized. BY-kinases have been shown to participate in various physiological processes. Nevertheless, we are at a very early stage of defining their importance in the bacterial cell. In Escherichia coli, two BY-kinases, Wzc and Etk, have been characterized biochemically. Wzc has been shown to phosphorylate the UDP-glucose dehydrogenase Ugd in vitro. Not only is Ugd involved in the biosynthesis of extracellular polysaccharides, but also in the production of UDP-4-amino-4-deoxy-L-arabinose, a compound that renders E. coli resistant to cationic antimicrobial peptides. Here, we studied the role of Ugd phosphorylation. We first confirmed in vivo the phosphorylation of Ugd by Wzc and we demonstrated that Ugd is also phosphorylated by Etk, the other BY-kinase identified in E. coli. Tyrosine 71 (Tyr71) was characterized as the Ugd site phosphorylated by both Wzc and Etk. The regulatory role of Tyr71 phosphorylation on Ugd activity was t...Continue Reading

References

Jul 1, 1991·Molecular Microbiology·S Gottesman, V Stout
Jan 1, 1980·Medical Microbiology and Immunology·J GoldharM Lahav
Nov 26, 1997·Journal of Bacteriology·E A GroismanF C Soncini
Dec 22, 1999·Trends in Cell Biology·C J Bakal, J E Davies
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Feb 27, 2001·Journal of Bacteriology·E A Groisman
Jan 5, 2002·Applied Microbiology and Biotechnology·D BergmaierC P Champagne
Mar 28, 2002·Journal of Molecular Biology·Detlef D LeipeL Aravind
Jul 26, 2002·The Journal of Biological Chemistry·Patricia DoubletAlain J Cozzone
Jan 10, 2003·Molecular Microbiology·Chakib Mouslim, Eduardo A Groisman
Mar 27, 2003·Molecular Microbiology·Gracjana KleinSatish Raina
Oct 22, 2003·Journal of Molecular Biology·Detlef D LeipeL Aravind
Jul 1, 2004·Molecular Microbiology·Yixin ShiEduardo A Groisman
Mar 3, 2005·Journal of Molecular Microbiology and Biotechnology·Ivan MijakovicJosef Deutscher
Jul 21, 2005·Journal of Bacteriology·Adi PelegIlan Rosenshine
Sep 13, 2005·Annual Review of Microbiology·Nadim Majdalani, Susan Gottesman
Feb 16, 2006·Molecular & Cellular Proteomics : MCP·Boris MacekMatthias Mann
Mar 22, 2006·Nucleic Acids Research·Ivan MijakovicDusica Vujaklija
Jun 8, 2006·Annual Review of Biochemistry·Chris Whitfield
Jul 4, 2006·Research in Microbiology·Soline LacourChristophe Grangeasse
Aug 9, 2006·Nature Reviews. Microbiology·Eduardo A Groisman, Chakib Mouslim
Jan 9, 2007·Trends in Biochemical Sciences·Christophe GrangeasseIvan Mijakovic
Jan 16, 2007·Molecular & Cellular Proteomics : MCP·Boris MacekMatthias Mann
Jan 18, 2007·The Journal of Biological Chemistry·Timothy C MeredithRonald W Woodard
Jan 27, 2007·Journal of Molecular Biology·Brice ObadiaChristophe Grangeasse
Mar 17, 2007·Annual Review of Biochemistry·Christian R H RaetzRussell E Bishop

❮ Previous
Next ❯

Citations

Dec 4, 2013·Antioxidants & Redox Signaling·Alistair J Standish, Renato Morona
Apr 15, 2014·Genome Biology and Evolution·Lei ShiIvan Mijakovic
Oct 3, 2009·Current Opinion in Infectious Diseases·Roger L Nation, Jian Li
Nov 11, 2011·International Journal of Molecular Sciences·Garry LavertyBrendan F Gilmore
Apr 18, 2013·Journal of Proteome Research·Nelson C SoaresBoris Macek
Aug 15, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christophe GrangeasseIvan Mijakovic
Oct 4, 2012·Expert Review of Anti-infective Therapy·Silpak BiswasJean-Marc Rolain
Jul 5, 2011·Proteomics·Boris Macek, Ivan Mijakovic
Sep 8, 2010·Molecular Microbiology·Taryn B Kiley, Nicola R Stanley-Wall
Apr 24, 2013·Bioscience Reports·Nicolás NavasaHonorina Martínez-Blanco
Nov 19, 2011·FEMS Microbiology Reviews·Ivan Mijakovic, Boris Macek
Dec 17, 2014·Frontiers in Microbiology·Abiola O OlaitanJean-Marc Rolain
Dec 1, 2015·BioMed Research International·Gracjana Klein, Satish Raina
Aug 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Luis A AlvarezUlla G Knaus
Dec 6, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Marco Salomone-StagniStefano Benini
Apr 2, 2013·The Journal of Biological Chemistry·Deniz B TemelRanajeet Ghose
Aug 10, 2016·Cold Spring Harbor Perspectives in Medicine·Michael J TrimbleRobert E W Hancock
Apr 28, 2020·FEBS Letters·Julie Bonne KøhlerIvan Mijakovic
Jul 14, 2020·Future Microbiology·Vijaya Bharathi Srinivasan, Govindan Rajamohan
Feb 5, 2021·Nature Communications·Leonardos MageirosSamuel K Sheppard
Apr 1, 2021·PLoS Pathogens·Alexandro Rodríguez-RojasJens Rolff
Aug 1, 2009·EcoSal Plus·Chris Whitfield
Jul 18, 2015·Microbiology Spectrum·Dominique H LimoliDaniel J Wozniak
Jul 18, 2021·Nature Communications·Yun YangJames H Naismith

❮ Previous
Next ❯

Methods Mentioned

BETA
phosphotransferase
dynamic light scattering
PCR

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bacterial Protein Structures (ASM)

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

© 2022 Meta ULC. All rights reserved