Tyrosine site-specific recombinases mediate DNA inversions affecting the expression of outer surface proteins of Bacteroides fragilis

Molecular Microbiology
Katja G WeinachtLaurie E Comstock

Abstract

The chromosome of Bacteroides fragilis has been shown to undergo 13 distinct DNA inversions affecting the expression of capsular polysaccharides and mediated by a serine site-specific recombinase designated Mpi. In this study, we demonstrate that members of the tyrosine site-specific recombinase family, conserved in B. fragilis, mediate additional DNA inversions of the B. fragilis genome. These DNA invertases flip promoter regions in their immediate downstream region. The genetic organization of the genes regulated by these invertible promoter regions suggests that they are operons and many of the products are predicted to be outer membrane proteins. Phenotypic analysis of a deletion mutant of one of these DNA invertases, tsr15 (aapI), which resulted in the promoter region for the downstream genes being locked ON, confirmed the synthesis of multiple surface proteins by this operon. In addition, this deletion mutant demonstrated an autoaggregative phenotype and showed significantly greater adherence than wild-type organisms in a biofilm assay, suggesting a possible functional role for these phase-variable outer surface proteins. This study demonstrates that DNA inversion is a universal mechanism used by this commensal microorgan...Continue Reading

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Citations

Jul 30, 2008·Antonie van Leeuwenhoek·Florence Wisniewski-Dyé, Ludovic Vial
Nov 2, 2005·Nature Reviews. Immunology·Gary W LitmanLarry J Dishaw
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michael J CoyneLaurie E Comstock
Dec 26, 2006·Journal of Bacteriology·Hazeline Roche-HakanssonLaurie E Comstock
Oct 30, 2012·Journal of Bacteriology·Ivan C NdamukongC Jeffrey Smith
Nov 13, 2007·Journal of Bacteriology·Michael J Coyne, Laurie E Comstock
Aug 20, 2008·Virology Journal·Shawn A HawkinsGary S Sayler
Dec 30, 2015·Trends in Microbiology·Marlena M Wilson, Harris D Bernstein
Nov 10, 2015·ACS Synthetic Biology·Jesús Fernández-RodríguezChristopher A Voigt
Apr 23, 2016·Cell·Michael J Coyne, Laurie E Comstock
Feb 10, 2016·PloS One·Haruyuki Nakayama-ImaohjiTomomi Kuwahara
Jan 9, 2019·Journal of Bacteriology·Jing-Wen LiJing-Ren Zhang
Aug 26, 2018·Scientific Reports·Travis N MavrichDouwe van Sinderen
Sep 21, 2020·Microbial Pathogenesis·Mina YekaniMohammad Yousef Memar

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