Abstract
The chromosome of Bacteroides fragilis has been shown to undergo 13 distinct DNA inversions affecting the expression of capsular polysaccharides and mediated by a serine site-specific recombinase designated Mpi. In this study, we demonstrate that members of the tyrosine site-specific recombinase family, conserved in B. fragilis, mediate additional DNA inversions of the B. fragilis genome. These DNA invertases flip promoter regions in their immediate downstream region. The genetic organization of the genes regulated by these invertible promoter regions suggests that they are operons and many of the products are predicted to be outer membrane proteins. Phenotypic analysis of a deletion mutant of one of these DNA invertases, tsr15 (aapI), which resulted in the promoter region for the downstream genes being locked ON, confirmed the synthesis of multiple surface proteins by this operon. In addition, this deletion mutant demonstrated an autoaggregative phenotype and showed significantly greater adherence than wild-type organisms in a biofilm assay, suggesting a possible functional role for these phase-variable outer surface proteins. This study demonstrates that DNA inversion is a universal mechanism used by this commensal microorgan...Continue Reading
References
Jul 1, 1992·Microbial Pathogenesis·J van DoornD M MacLaren
Mar 1, 1992·Plasmid·C J SmithM L McKee
Jan 1, 1991·Proteins·K Nakai, M Kanehisa
Jun 1, 1991·Infection and Immunity·A PantostiD L Kasper
May 1, 1990·Journal of Bacteriology·J S Thompson, M H Malamy
Aug 1, 1990·Journal of Bacteriology·A M StevensA A Salyers
Jun 22, 1999·Infection and Immunity·L E ComstockD L Kasper
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·L V HooperJ I Gordon
Aug 10, 2000·Molecular Microbiology·P N DaneseR Kolter
Oct 18, 2000·Infection and Immunity·M J CoyneL E Comstock
Nov 30, 2000·FEMS Microbiology Letters·D P BayleyC J Smith
Dec 6, 2001·Nature·C M KrinosL E Comstock
Nov 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Thaddeus S StappenbeckJeffrey I Gordon
Apr 11, 2003·Microbiology·Sheila PatrickGarry Blakely
Jul 24, 2003·Protein Science : a Publication of the Protein Society·Agnieszka S JunckerAnders Krogh
Aug 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael J CoyneLaurie E Comstock
Dec 24, 2003·Nature Immunology·Denise KellyShaun Conway
Citations
Jan 12, 2008·Microbial Ecology·Lilian PumbweHannah M Wexler
Jul 30, 2008·Antonie van Leeuwenhoek·Florence Wisniewski-Dyé, Ludovic Vial
Nov 2, 2005·Nature Reviews. Immunology·Gary W LitmanLarry J Dishaw
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michael J CoyneLaurie E Comstock
Aug 4, 2009·Journal of Bacteriology·Haruyuki Nakayama-ImaohjiTomomi Kuwahara
Dec 26, 2006·Journal of Bacteriology·Hazeline Roche-HakanssonLaurie E Comstock
Oct 30, 2012·Journal of Bacteriology·Ivan C NdamukongC Jeffrey Smith
Nov 28, 2007·Journal of Bacteriology·Maria Chatzidaki-LivanisLaurie E Comstock
Nov 13, 2007·Journal of Bacteriology·Michael J Coyne, Laurie E Comstock
Jul 21, 2006·Journal of Bacteriology·Mary E Davey, Margaret J Duncan
Aug 20, 2008·Virology Journal·Shawn A HawkinsGary S Sayler
Dec 30, 2015·Trends in Microbiology·Marlena M Wilson, Harris D Bernstein
Nov 10, 2015·ACS Synthetic Biology·Jesús Fernández-RodríguezChristopher A Voigt
May 26, 2007·Microbial Pathogenesis·Lilian PumbweHannah M Wexler
Apr 22, 2009·Cell·C Mark FletcherLaurie E Comstock
Feb 7, 2015·PloS One·Marlena M WilsonHarris D Bernstein
Apr 23, 2016·Cell·Michael J Coyne, Laurie E Comstock
Oct 1, 2015·MBio·Mao TaketaniMichael A Fischbach
Feb 10, 2016·PloS One·Haruyuki Nakayama-ImaohjiTomomi Kuwahara
Feb 18, 2011·Gut Microbes·Richard A StablerBrendan W Wren
Jan 9, 2019·Journal of Bacteriology·Jing-Wen LiJing-Ren Zhang
Jun 28, 2019·Frontiers in Microbiology·Hui YuZhanli Wang
Aug 26, 2018·Scientific Reports·Travis N MavrichDouwe van Sinderen
Sep 21, 2020·Microbial Pathogenesis·Mina YekaniMohammad Yousef Memar
Oct 7, 2021·BMC Microbiology·Julia IsenringMarc J A Stevens