Tyrosine-specific phosphorylation of calmodulin by the insulin receptor kinase purified from human placenta.

The Biochemical Journal
D B SacksJ M McDonald

Abstract

It has previously been demonstrated that calmodulin can be phosphorylated in vitro and in vivo by both tyrosine-specific and serine/threonine protein kinase. We demonstrate here that the insulin receptor tyrosine kinase purified from human placenta phosphorylates calmodulin. The highly purified receptors (prepared by insulin-Sepharose chromatography) were 5-10 times more effective in catalysing the phosphorylation of calmodulin than an equal number of partially purified receptors (prepared by wheat-germ agglutinin-Sepharose chromatography). Phosphorylation occurred exclusively on tyrosine residues, up to a maximum of 1 mol [0.90 +/- 0.14 (n = 5)] of phosphate incorporated/mol of calmodulin. Phosphorylation of calmodulin was dependent on the presence of certain basic proteins and divalent cations. Some of these basic proteins, i.e. polylysine, polyarginine, polyornithine, protamine sulphate and histones H1 and H2B, were also able to stimulate the phosphorylation of calmodulin via an insulin-independent activation of the receptor tyrosine kinase. Addition of insulin further increased incorporation of 32P into calmodulin. The magnitude of the effect of insulin was dependent on the concentration and type of basic protein used, rang...Continue Reading

Citations

Mar 19, 2014·Protein & Cell·Yipeng Du, Taotao Wei
Dec 28, 2010·Biochimica Et Biophysica Acta·Colin D WhiteDavid B Sacks
Sep 26, 2014·ELife·Jennifer M KavranDaniel J Leahy
Feb 26, 2000·Journal of Neuroendocrinology·H CastelH Vaudry
Dec 29, 2000·American Journal of Veterinary Research·R Paxton, L X Ye
Mar 1, 1995·Journal of Cellular Biochemistry·J P WilliamsJ M McDonald
Sep 15, 1994·European Journal of Biochemistry·A BenguríaA Villalobo
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Nov 15, 1995·European Journal of Biochemistry·A BenguríaA Villalobo
Mar 30, 2001·The Journal of Biological Chemistry·Y V MukhinM N Garnovskaya
Feb 9, 2017·The Journal of Biological Chemistry·Zhigang LiDavid B Sacks
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Aug 3, 2002·European Journal of Biochemistry·Gustavo Benaim, Antonio Villalobo
Jul 1, 2001·The New Phytologist·Wayne A Snedden, Hillel Fromm
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Jan 1, 1994·Molecular Aspects of Medicine·G Desoye, E Shafrir
Dec 10, 1996·Biochemistry·H G MunshiD B Sacks

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