PMID: 2119315Sep 3, 1990Paper

Tyrosine-specific phosphorylation of gpIIIa in platelet membranes

FEBS Letters
M A ElmoreS Kellie

Abstract

In vitro phosphorylation of platelet subcellular fractions revealed that most of the alkali-resistant phosphoproteins and the majority of pp60c-src were in the surface membrane fraction. An alkali-resistant phosphoprotein of about 100 kDa was also immune precipitated by an anti-phosphotyrosine antibody and comigrated with gpIIIa. The phosphorylation of gpIIIa, but not gpIIb, was confirmed by the comparison of reduced and non-reduced gels, and this protein was phosphorylated exclusively on tyrosine. In contrast, both gpIIb and gpIIIa were phosphorylated when the purified complex was added to immunopurified, immobilised pp60c-src. A synthetic peptide with partial homology to a putative tyrosine phosphorylation site in the cytoplasmic domain of gpIIIa was phosphorylated by antibody-purified pp60c-src. Our results indicate that tyrosine-specific phosphorylation of gpIIIa by pp60c-src may play a role in the regulation of platelet function.

References

Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A GoldenJ S Brugge
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Golden, J S Brugge
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E Ferrell, G S Martin
Jan 1, 1988·Annual Review of Biochemistry·E Ruoslahti
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R HirstL Rohrschneider
Feb 24, 1988·Biochimica Et Biophysica Acta·K J Clemetson, E F Lüscher
Feb 1, 1981·Molecular and Cellular Biology·J A Cooper, T Hunter

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Citations

May 1, 1993·British Journal of Haematology·A Dhar, S D Shukla
Jan 1, 1992·Platelets·P Harrison
Apr 12, 2002·Oncogene·Brigitte BoyerMarie-France Poupon
Oct 1, 1991·Current Opinion in Cell Biology·S J Shattil, J S Brugge

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