U-13C]glutamate metabolism in rat brain mitochondria reveals malic enzyme activity

Neuroreport
I J BakkenT E Bates

Abstract

13C nuclear magnetic resonance spectroscopy was used to study the activity of malic enzyme in isolated brain mitochondria from rat in the presence of unlabelled malate and [U-13C]glutamate. ADP, inorganic phosphate, malate and [U-13C]glutamate were added to a suspension of oxygenated mitochondria. Typical tricarboxylic acid (TCA) cycle constituents (malate, 2-oxoglutarate and succinate) were labelled from [U-13C]glutamate and detected in the superfusion medium. The labelling patterns in the different atom positions of glutamate revealed entry of both unlabelled and labelled acetyl-CoA into the TCA cycle. Unlabelled acetyl-CoA was derived via pyruvate from exogenously applied malate by the action of mitochondrial malic enzyme, while labelled acetyl-CoA was derived from TCA cycle intermediates, most likely by the action of mitochondrial malic enzyme on malate produced from [U-13C]glutamate. The results demonstrate malic enzyme activity and pyruvate recycling in isolated rat brain mitochondria.

References

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Citations

Jun 25, 2014·Metabolites·Simon-Pierre GravelJulie St-Pierre
Sep 12, 2007·Journal of Neuroscience Research·Mary C McKenna
Jul 30, 2015·Glia·Jakob D NissenHelle S Waagepetersen
Nov 15, 2006·The Journal of Biological Chemistry·Rebecca L PongratzGary W Cline
Feb 17, 2007·Journal of Neuroscience Research·Elisabeth OlstadUrsula Sonnewald

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