Apr 11, 1984

U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle

Nucleic Acids Research
C Hashimoto, J A Steitz


U4 and U6 RNAs of mammalian cells possess extensive intermolecular sequence complementarity and hence have the potential to base pair. A U4/U6 RNA complex, detectable in nondenaturing polyacrylamide gels, is released when human small nuclear ribonucleoproteins (snRNPs) containing U1, U2, U4, U5, and U6 RNAs are dissociated with proteinase K in the presence of sodium dodecyl sulfate. The released RNA/RNA complex dissociates with increasing temperature, consistent with the existence of specific base-pairing between the two RNAs. Since U6 RNA is selectively released from intact snRNPs under the same conditions required to dissociate the U4/U6 RNA complex, the RNA-RNA interaction may be sufficient to maintain U4 and U6 RNAs in the same snRNP particle. The biological implications of these findings are discussed.

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Mentioned in this Paper

Small Nuclear RNA
RNA Conformation
Polyacrylamide Gels
Small Nuclear Ribonucleoproteins
U6 small nuclear RNA
Small Nuclear Ribonucleoprotein Complex Location
Base Pairing
Small ribonucleoprotein antibody
HeLa Cells

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