U6 can be used as a housekeeping gene for urinary sediment miRNA studies of IgA nephropathy

Scientific Reports
Zhi-Yu DuanXiang-Mei Chen

Abstract

Recent studies have indicated that urinary sediment miRNAs not only are able to serve as non-invasive diagnostic biomarkers for IgA nephropathy (IgAN) but may also be closely related to several clinical and pathological indicators. However, the lack of a suitable internal reference miRNA has hampered research into urinary sediment miRNAs. To date, U6 has been used as a reference gene in urinary sediment miRNA studies mostly based on the results from studies using tissue samples and cell lines. In a total of 330 IgAN patients, 164 disease control patients and 130 normal control patients, there was no significant difference in U6 levels. We also compared the U6 levels in different types of primary glomerular disease groups (IgA nephropathy, membranous nephropathy, minimal change nephrosis and focal segmental glomerular sclerosis). The results confirmed that there was no significant difference in the expression of U6 in different primary glomerular disease groups. Moreover, treatment had no significant effect on the expression levels of U6 in IgA nephropathy. Therefore, U6 is an excellent housekeeping gene for urinary sediment miRNA studies of IgA nephropathy.

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M R Lerner, J A Steitz
Oct 1, 1993·Genes & Development·M P TernsE Lund
May 3, 1996·The Journal of Biological Chemistry·S B NoonbergC C Benz
Sep 6, 2002·The New England Journal of Medicine·James V Donadio, Joseph P Grande
Jan 6, 2009·Cytokine·Ananthi J AsirvathamThomas B Tomasi
Jun 13, 2009·American Journal of Nephrology·Guang-Yan Cai, Xiang-Mei Chen
Nov 19, 2011·Nature Reviews. Genetics·Manel Esteller
Mar 1, 2012·Journal of the American Society of Nephrology : JASN·Grazia SerinoFrancesco P Schena
Sep 11, 2012·Disease Markers·Cheuk-Chun SzetoLi Philip Kam-Tao
Jan 15, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gang WangCheuk-Chun Szeto
Jan 29, 2014·Biochemical and Biophysical Research Communications·Hao BaoZhihong Liu
Apr 9, 2014·Nature Reviews. Nephrology·Cheuk-Chun Szeto, Philip K-T Li
Mar 24, 2016·Scientific Reports·Zhi-Yu DuanXiang-Mei Chen
Jan 26, 2018·RNA·Allison L DidychukDavid A Brow

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Citations

Sep 29, 2019·Molecular Aspects of Medicine·Guo-Liang HuangGeorge A Calin
Mar 26, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Baozhen SunLianyue Guan

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Methods Mentioned

BETA
glycosylation
immunoprecipitation
biopsy

Software Mentioned

GraphPad
GraphPad Prism
SPSS

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