U6 snRNA intron insertion occurred multiple times during fungi evolution

RNA Biology
Sebastian CanzlerJana Hertel

Abstract

U6 small nuclear RNAs are part of the splicing machinery. They exhibit several unique features setting them appart from other snRNAs. Reports of introns in structured non-coding RNAs have been very rare. U6 genes, however, were found to be interrupted by an intron in several Schizosaccharomyces species and in 2 Basidiomycota. We conducted a homology search across 147 currently available fungal genome and identified the U6 genes in all but 2 of them. A detailed comparison of their sequences and predicted secondary structures showed that intron insertion events in the U6 snRNA were much more common in the fungal lineage than previously thought. Their positional distribution across the entire mature snRNA strongly suggests a large number of independent events. All the intron sequences reported here show canonical splice site and branch site motifs indicating that they require the splicesomal pathway for their removal.

References

Oct 11, 1989·Nucleic Acids Research·J Potashkin, D Frendewey
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R Singh, R Reddy
Oct 1, 1988·Genes & Development·R Reed, T Maniatis
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G R KunkelT Pederson
Apr 11, 1984·Nucleic Acids Research·C Hashimoto, J A Steitz
Dec 24, 1993·Science·S Kandels-Lewis, B Séraphin
Sep 1, 1993·Molecular and Cellular Biology·Y TakahashiY Ohshima
Oct 8, 2004·Eukaryotic Cell·Doris M KupferJuneann W Murphy
Jan 20, 2009·Nucleic Acids Research·Jana HertelPeter F Stadler
Oct 31, 2009·RNA Biology·Manja Marz, Peter F Stadler
Nov 6, 2010·Nucleic Acids Research·Philip WongUlrich Güldener
Jan 24, 2014·Nature Reviews. Molecular Cell Biology·A Gregory Matera, Zefeng Wang
Nov 14, 2014·Nucleic Acids Research·Eric P NawrockiRobert D Finn

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq

Software Mentioned

BLAST
GotohScan
FIMO
MEME

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.