UAP56 is an important regulator of protein synthesis and growth in cardiomyocytes.

Biochemical and Biophysical Research Communications
Abha SahniJeffrey D Alexis

Abstract

UAP56, an ATP dependent RNA helicase that also has ATPase activity, is a DExD/H box protein that is phylogenetically grouped with the eukaryotic initiation factor eIF4A, the prototypical member of the DExD/H box family of helicases. UAP56, also known as BAT1, is an essential RNA splicing factor required for spliceosome assembly and mRNA export but its role in protein synthesis is not known. Here we demonstrate that UAP56 regulates protein synthesis and growth in cardiomyocytes. We found that wild-type (WT) UAP56 increased serum induced protein synthesis in HeLa cells. UAP56 mutants lacking ATPase and/or helicase activity inhibited protein synthesis compared with WT UAP56, suggesting that the ATPase and RNA helicase activity of UAP56 is important for protein synthesis. UAP56 siRNA inhibited phenylephrine (PE) induced protein synthesis in cardiomyocytes and inhibited PE induced cardiomyocyte hypertrophy. Our data demonstrate that UAP56 is an important regulator of protein synthesis and plays an important role in the regulation of cardiomyocyte growth.

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Citations

Jul 19, 2012·In Vitro Cellular & Developmental Biology. Animal·Jian-Guo HuangXin Jin
Aug 24, 2011·The Journal of Biological Chemistry·Christian WisskirchenJovan Pavlovic
Oct 8, 2013·BMC Complementary and Alternative Medicine·Nurhuda Mohamad AnsorNorhaniza Aminudin
Mar 13, 2012·Annual Review of Biophysics·Arnon HennEnrique M De La Cruz
Mar 27, 2012·Biochemical and Biophysical Research Communications·Abha SahniJeffrey D Alexis
Aug 21, 2016·Molecular Microbiology·Mariana SerpeloniAndréa R Ávila
Sep 4, 2018·RNA Biology·Sharad AwasthiArunkumar Dhayalan
Aug 23, 2018·Molecular Medicine Reports·Bin NiShanxin Liu
Feb 26, 2020·Endocrine, Metabolic & Immune Disorders Drug Targets·Yuhong DuanChunguang Xie

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