Ubc9 interacts with SOX4 and represses its transcriptional activity

Biochemical and Biophysical Research Communications
Xin PanXuemin Zhang

Abstract

SOX4 is a member of SOX transcriptional factor family that is crucial for many cellular processes. In this study, a yeast two-hybrid screening of human mammary cDNA library identified human ubiquitin-conjugating enzyme 9 (hUbc9) that interacted with SOX4. This interaction was confirmed by GST pull-down in vitro and co-immunoprecipitation assays in vivo. Deletion mapping demonstrated that HMG-box domain of SOX4 is required to mediate the interaction with Ubc9 in yeast. Furthermore, confocal microscopy showed that Ubc9 co-localized with SOX4 in the nucleus. Luciferase assays found that Ubc9 specifically repressed SOX4 transcriptional activity in 293T cells. We further demonstrated that Ubc9 could functionally repress the transcriptional activity of endogenous SOX4 induced by progesterone in T47D cells. The C93S mutant of Ubc9, which abrogates SUMO-1 conjugation activity, did not abolish the ability to repress SOX4 activity. It shows that Ubc9 interacts with SOX4 and represses its transcriptional activity independent of its SUMO-1-conjugating activity.

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Citations

Mar 26, 2008·Nature Immunology·Hui-Yan LiXue-Min Zhang
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xin PanXue-Min Zhang
Nov 11, 2011·Journal of Virology·Peter WimmerThomas Dobner
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Oct 30, 2014·ELife·Sen-Lin Lai, Chris Q Doe
Aug 31, 2017·Neural Regeneration Research·Kun-Che Chang, Jonathan Hertz

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