Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy

Journal of Hematology & Oncology
Tong ShenJian-Ming Li

Abstract

Ubiquitination is a basic post-translational modification for cellular homeostasis, and members of the conjugating enzyme (E2) family are the key components of the ubiquitin-proteasome system. However, the role of E2 family in colorectal cancer (CRC) is largely unknown. Our study aimed to investigate the role of Ube2v1, one of the ubiquitin-conjugating E2 enzyme variant proteins (Ube2v) but without the conserved cysteine residue required for the catalytic activity of E2s, in CRC. Immunohistochemistry and real-time RT-PCR were used to study the expressions of Ube2v1 at protein and mRNA levels in CRC, respectively. Western blotting and immunofluorescence, transmission electron microscopy, and in vivo rescue experiments were used to study the functional effects of Ube2v1 on autophagy and EMT program. Quantitative mass spectrometry, immunoprecipitation, ubiquitination assay, western blotting, and real-time RT-PCR were used to analyze the effects of Ube2v1 on histone H4 lysine 16 acetylation, interaction with Sirt1, ubiquitination of Sirt1, and autophagy-related gene expression. Ube2v1 was elevated in CRC samples, and its increased expression was correlated with poorer survival of CRC patients. Ube2v1 promoted migration and invasion...Continue Reading

References

Jul 19, 2001·Nature·C WangZ J Chen
Jan 7, 2004·Cancer Cell·Aimee L Edinger, Craig B Thompson
Sep 30, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Angelika M Burger, Arun K Seth
Aug 22, 2007·The Journal of Biological Chemistry·Matthew D PetroskiJianing Huang
Nov 2, 2007·Nature Reviews. Cancer·Robin MathewEileen White
May 20, 2009·Molecular Cell·Vladimir KirkinIvan Dikic
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Jun 16, 2009·Cell·Jorge Moscat, Maria T Diaz-Meco
Jun 16, 2009·Cell·Robin MathewEileen White
Aug 6, 2009·Annual Review of Genetics·Congcong He, Daniel J Klionsky
Aug 14, 2009·Nature·Zong-Ping XiaZhijian J Chen
Jan 9, 2010·Current Opinion in Cell Biology·Eileen WhiteRobin Mathew
Sep 3, 2010·Nature Cell Biology·Claudine KraftKay Hofmann
Mar 17, 2011·Genes & Development·Shenghong YangAlec C Kimmelman
Mar 1, 2012·Nature·Reuven WienerCynthia Wolberger
Apr 27, 2012·Nature Reviews. Cancer·Eileen White
May 25, 2013·Journal of the National Cancer Institute·Lina SunYong Xu
Aug 13, 2013·Autophagy·Jens FüllgrabeBertrand Joseph
Dec 5, 2013·Autophagy·He LiuHans-Uwe Simon
Dec 10, 2013·Cell·Jessie Yanxiang GuoEileen White
Feb 6, 2015·The Journal of Clinical Investigation·Eileen White
Feb 26, 2015·The EMBO Journal·Lorenzo GalluzziGuido Kroemer
May 30, 2015·Molecular Oncology·Myriam CatalanoSabrina Di Bartolomeo

❮ Previous
Next ❯

Citations

May 10, 2019·Cancer Cell International·Shentong YuJing Zhang
Apr 26, 2019·Journal of Cellular Physiology·Saleheh RezapourAli Hassanzadeh
Jan 10, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhe WangMin Hao
Jul 1, 2020·Recent Patents on Anti-cancer Drug Discovery·Javad Saffari-ChaleshtoriSayed Mohammad Shafiee
Dec 2, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Jared DeyarminJane Khudyakov
Oct 14, 2019·Archives of Biochemistry and Biophysics·Zheng WuLiang Yuan
Apr 4, 2021·International Journal of Molecular Sciences·Xiaodi DuGuangyou Yang
Nov 28, 2020·Pharmacology & Therapeutics·Michele AventaggiatoMarco Tafani
Sep 2, 2021·Biochemistry and Biophysics Reports·Zhishuai RenYuqi Guo
Nov 30, 2021·Journal of Cardiovascular Pharmacology·Musammat Kulsuma BegumYu Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
ubiquitination
PCR
biopsies
fluorescence microscopy
Protein Assay
co-immunoprecipitation
Immunoprecipitation
xenograft

Software Mentioned

con7trast
PrognoScan
Proteome Discovery
Mascot

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms