Ubiquinone (coenzyme Q10) binding sites: low dielectric constant of the gate prevents the escape of the semiquinone

FEBS Letters
V P Shinkarev

Abstract

The photosynthetic reaction center (RC) from purple bacteria is frequently used as a model for the interaction of ubiquinones (coenzyme Q) with membrane proteins. Single-turnover flash activation of RC leads to formation of the semiquinone (SQ) of the secondary acceptor quinone after odd flashes and quinol after even flashes. The ubiquinol escapes the binding site in 1 ms, while the SQ does not leave the binding site for at least 5 min. Observed difference between these times suggests a large energetic barrier for the SQ. However, high apparent dielectric constant in the vicinity of the quinone ring (>or=25) results in a relatively small electrostatic energy of SQ stabilization. To resolve this apparent contradiction I suggest that a significant part of the kinetic stabilization of the SQ is achieved by the special topology of the binding site in which quinone can exit the binding site only by moving its headgroup toward the center of the membrane. The large energetic penalty of transferring the charged headgroup to the membrane dielectric can explain the observed kinetic stability of the SQ.

References

Mar 13, 1985·Biochimica Et Biophysica Acta·R J Shopes, C A Wraight
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J P AllenD C Rees
Apr 9, 1984·Biochimica Et Biophysica Acta·P R Rich
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Mar 26, 1998·Biochimica Et Biophysica Acta·D P TielemanH J Berendsen
Feb 25, 2000·Journal of Molecular Biology·N Fisher, P R Rich
May 17, 2000·Biochimica Et Biophysica Acta·M Y OkamuraG Feher
Aug 31, 2000·Annual Review of Biochemistry·E A BerryA R Crofts
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Günter FritzschLaura Baciou
Jul 9, 2003·Annals of the New York Academy of Sciences·M Flint Beal
Feb 7, 2004·Science·Kristina N FerreiraSo Iwata
Mar 25, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Asif Rahaman, Ralph A Wheeler
Apr 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Richard H G BaxterJames R Norris
Jun 24, 2004·Journal of the American Chemical Society·Hiroshi Ishikita, Ernst-Walter Knapp
Apr 29, 2005·Acta Crystallographica. Section D, Biological Crystallography·Richard H G BaxterJames R Norris
Aug 17, 2005·Biochemistry·Jennifer Madeo, M R Gunner
Oct 6, 2005·Current Opinion in Clinical Nutrition and Metabolic Care·Gian Paolo Littarru, Luca Tiano
Oct 19, 2005·Genes & Development·Hugo AguilaniuAndrew Dillin
Jan 3, 2006·Biochimica Et Biophysica Acta·Vladimir P ShinkarevColin A Wraight

❮ Previous
Next ❯

Citations

Jul 20, 2007·Photosynthesis Research·Jan Kern, Gernot Renger
May 4, 2013·Annual Review of Plant Biology·Anthony L MooreKikukatsu Ito
Oct 9, 2015·European Biophysics Journal : EBJ·Olga P Kaminskaya, Vladimir A Shuvalov
Mar 31, 2016·Doklady. Biochemistry and Biophysics·O P Kaminskaya, V A Shuvalov
Oct 7, 2016·Photosynthesis Research·Tibor SzabóLászló Nagy
Sep 7, 2013·Chembiochem : a European Journal of Chemical Biology·Preethi R VennamMichael K Bowman
Sep 2, 2009·Physiologia Plantarum·Mary S AlburyAnthony L Moore

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.