PMID: 672Oct 1, 1975

Ubiquinone-mediated coupling of NADH dehydrogenase to active transport in membrane vesicles from Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
P Stroobant, H R Kaback

Abstract

Addition of ubiquinone-1 to E. coli ML 308-225 membrane vesicles dramatically increases coupling between NADH oxidation and active transport such that initial rates and steady-state levels of lactose and amino-acid accumulation are comparable to those observed during D-lactate oxidation. Similar but less dramatic effects are observed with the quinone and succinate or L-lactate. In the presence of NADH and ubiquinone-1, the vesicles also generate a membrane potential (interior negative) that is similar in magnitude to that observed in the presence of D-lactate. Stimulation of NADH-dependent transport by ubiquinone-1 cannot be accounted for by increased rates of oxidation of NADH, and the effect of the quinone on NADH-dependent lactose transport is not observed in vesicles depleted of NADH dehydrogenase activity. Thus, it is apparent that ubiquinone-1 shunts electrons from NADH dehydrogenase [NADH:(acceptor)oxidoreductase; EC 1.6.99.3] to the portion of the respiratory chain containing the energy-coupling site. The findings demonstrate unequivocally that inefficient coupling of NADH oxidation to active transport cannot be due to the presence of inverted vesicles. In addition, they provide further support for specific localization...Continue Reading

References

Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J F HareE P Kennedy
Oct 25, 1973·Biochimica Et Biophysica Acta·G van Thienen, P W Postma
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·H R Kaback, L S Milner
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·B P Rosen, J S McClees
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H HirataF M Harold
Aug 4, 1972·Biochimica Et Biophysica Acta·H R Kaback
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J P ReevesH R Kaback
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W N Konings, H R Kaback
Jan 1, 1974·The Journal of Membrane Biology·J H Weiner
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S A ShortS C Silverstein
Dec 6, 1974·Science·H R Kaback
Dec 23, 1969·Journal of Chromatography·R K Hammond, D C White

Citations

Jan 1, 1977·Journal of Supramolecular Structure·H R KabackH Tokuda
Nov 18, 1977·Archives of Microbiology·L DijkhuizenW N Konings
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S RamosH R Kaback
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P Owen, H R Kaback
Feb 15, 1983·European Journal of Biochemistry·M T Davison, P B Garland
Jan 1, 1980·Annals of the New York Academy of Sciences·H R Kaback
Apr 17, 1979·Biochemistry·P Owen, H R Kaback
Dec 1, 1976·Journal of Cellular Physiology·H R Kaback
Dec 1, 1981·European Journal of Biochemistry·J BergsmaW N Konings

Related Concepts

Plasma Membrane
Alkalescens-Dispar Group
Lactates
Anhydrous lactose
NADH
NADH, NADPH Oxidoreductases
NADP
Oxygen Consumption
Succinic Acids
Ubiquinone

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